EAGER: The International CHO Genome Community Project

这项热切的提议的总体目标是开发一个框架，以支持国际中国仓鼠卵巢(CHO)细胞社区进入基因组时代的努力。CHO细胞是目前应用最广泛的哺乳动物细胞系，用于生物制药生产。在全球每年产生近1000亿美元市场的所有重组生物疗法中，近70%是在CHO细胞中生产的。生物制药生产将在未来几年继续为制药业提供巨大的增长，使人们能够继续研究新的医疗方法并扩大制造设施。不幸的是，由于缺乏基因组和社区基因组框架，使用代谢工程和其他科学和工程方法改进该系统的努力一直滞后。因此，该项目将实施基础设施，以实现支持国际CHO社区的两个具体目标：具体目标1：开发信息技术(IT)基础设施，以支持CHO生物技术社区共享基因组规模的数据和工具，并提供一个CHO生物技术社区可以在线互动和协同的论坛。具体目标2：教育研究生使用生物信息学来实施和分析CHO基因组，并使用对CHO社区重要的新网络工具提高特定的生产力和产品质量。这项提议的智力价值来自于开发一个一站式网站门户网站来支持社区。作为建立这个门户的一部分，将需要使用一些生物信息学工具来将现有的基因组规模的数据集无缝地迁移到一个公共界面中。这项提议的更广泛的影响涉及到这一努力将对整个国际CHO生物技术社区产生的重要影响。来自包括学术、政府和工业科学家在内的广泛国际实体的合作伙伴已经走到一起，通过提供资源、专业知识和数据来支持CHO基因组社区。其他影响将来自为该项目工作的研究生的教育，他们将与工业科学家密切联系，并从工业界的劳动力发展活动中受益。

The overall objective for this EAGER proposal is to develop a framework to support the international Chinese hamster ovary (CHO) cell community's effort to move into the genomeera. CHO cells are currently the most widely used mammalian cell line for biopharmaceutical production. Nearly 70% of all recombinant biotherapeutics, yielding a global market of nearly $100 billion per year, are produced in CHO cells. Biopharmaceutical production will continue to provide the pharmaceutical industry with tremendous growth in the next several years, enabling continued research into new medical treatments and the expansion of manufacturing facilities. Unfortunately, efforts to improve the system using metabolic engineering and other scientific and engineering methodologies have lagged due to the absence of a genome and communitygenome framework. As a result, this project will implement the infrastructure to pursue two specific aims for support of the international CHO community:Specific Aim #1:Develop an information technology (IT) - infrastructure to support the sharing of genome-scale data and tools for the CHO biotechnology community and provide a forum where the CHO biotechnology community can interact and synergize on-line.Specific Aim #2:Educate graduate students on the use of bioinformatics to implement and analyze the CHO genome and enhance specific productivity and product quality using new webbased tools of importance to the CHO community.The intellectual merit of this proposal results from the development of a one-stop website portal to support the community. As part of the establishment of this portal, a number of bioinformatic tools will need to be employed to seamlessly migrate existing genome-scale datasets into a common interface.The broader impact of this proposal relates to the important impact this effort will have on the entire international CHO biotechnology community. Partners from a wide range of international entities including academic, government, and industrial scientists, have come together to support the CHO genome community by providing resources, expertise, and data. Additional impact will come from the education of graduate students working on the project who will closely interface with industrial scientists and from workforce development activities for the industrial community.