MRI: Acquisition of a Mass Spectrometer

圣克拉拉大学的Michael Carrasco教授和同事Amelia Fuller将获得主要研究仪器（MRI）项目的这项奖励，他们将获得一套电喷雾化串联（ESI-MS/MS）质谱系统。该奖项将加强各级的研究培训和教育，特别是在以下领域：(a)鉴定模拟蛋白质的折叠体，(b)防止蛋白质水解的肽的化学选择性糖基化，(c)糖肽肽的合成，(d)糖肽和糖蛋白的适体选择，(e)鉴定从北realis癌症心包器官释放的生物源胺。(f)蛋白质与纳米银之间相互作用的表征，以及(g)卟啉组合的配体结合特性的鉴定。质谱仪（MS）是通过测量样品中分子组分电离后经质谱仪检测后的质量，来鉴定样品的化学成分。该仪器将高分辨率液相色谱系统与质谱的质量分析能力相结合。位于光谱仪口的液相色谱仪将混合物分离成它们的分子成分。然后这些成分流入质谱仪，在那里它们被电喷雾源电离。串联质谱计（MS/MS）涉及多个步骤，在各阶段之间会发生某种形式的分裂。这样就可以测量它们的质量和碎片的质量。该仪器不仅将用于研究，而且还将用于实验室课程，以训练大量学生使用这一重要的分析技术。

With this award from the Major Research Instrumentation (MRI) program, Professor Michael Carrasco from Santa Clara University and colleague Amelia Fuller will acquire an electrospray inonization tandem (ESI-MS/MS) mass spectrometric system. The award will enhance research training and education at all levels, especially in areas such as (a) identification of foldamers that mimic proteins, (b) chemoselective glycosylation of peptides that protection from proteolysis, (c) synthesis of glycolipopeptides, (d) aptamer selection for glycopeptides and glycoproteins, (e) characterization of release of biogenic amines from the pericardial organs of cancer borealis, (f) characterization of interactions between proteins and silver nanoparticles, and (g) identification of the ligand-binding properties of porphyrin assemblies.Mass spectrometers (MS) are used to identify the chemical composition of a sample by measuring the mass of the molecular constituents in the sample after they are ionized and detected by the mass spectrometer. This instrument couples a high-resolution liquid chromatography system with the mass analysis ability of mass spectrometry. A liquid chromatograph located at the mouth of the spectrometer separates mixtures into their molecular components. These components then flow into the mass spectrometer where they are ionized using an electrospay source. The tandem mass spectrometer (MS/MS) involves multiple steps with some form of fragmentation occurring between stages. In this way their masses, and those of their fragments, are measured. The instrumentation will be used not only for research but also in laboratory courses to train significant numbers of students in the use of this important analytical technique.