CAREER: Ultra- Sensitive and Accurate Quantification of Protein Carbonyl Formation using Ratiometric Cleavable Surface-Enhanced Raman Tags (RCST)

在化学系化学测量和成像（CMI）项目的支持下，以及刺激竞争性研究实验项目办公室（EPSCoR）的共同资助下，密西西比州立大学（MSU）的张东茂教授及其团队正在探索和开发一种用于定量蛋白质羰基的创新，准确，超灵敏和可重复的分析方法，被广泛接受的蛋白质氧化损伤的生物标志物。本工作的具体目标是：1）设计合成一对罗丹明染料衍生物，用于蛋白质羰基的Sers标记。 2)测定基于Sers的蛋白质羰基定量技术的灵敏度、准确度、再现性和动态范围。 3)肽和蛋白质的结合对标记染料的吸附、聚集和Sers活性的影响的实验和计算研究。 此外，Sers方法的灵敏度，准确性和再现性将与两种最流行的生物分析方法：质谱法和荧光法进行比较。 该提案的主要教育目标是：1）继续在张博士的实验室中吸引和指导来自密西西比州立大学和（PI的家乡大学）和密西西比女子大学，附近的非博士授予机构，2）通过与附近的高中合作，帮助吸引密西西比高中学生进入STEM领域，该高中的辍学率很高-低的大学入学率。 通过参与拟议的研究，高中生，本科生和研究生将获得在纳米粒子界面相互作用和光谱学领域的宝贵经验。 为了加强合作高中的化学教育，PI及其团队将协助合作高中化学教师建立教学实验室，设计和提供化学实验。 PI实验室的本科生和高中生研究人员将与合作高中的同龄人分享他们的大学和研究经验，以激励高中生追求STEM领域的职业生涯。 从这个长期的大学/高中伙伴关系的学习将通过全国会议和/或期刊出版物在全国范围内共享。 张博士希望激发更多的创造性想法，以促进欠发达地区的教育。 该奖项支持的研究和教育活动的进展将在PI的研究网站http://www.chemistry.msstate.edu/people/faculty/detail.php?上公布教师=zhang

With support from the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry, and co-funding from the Office of Experimental Program to Stimulate Competitive Research (EPSCoR), Professor Dongmao Zhang and his group at Mississippi State University (MSU) are exploring and developing an innovative, accurate, ultra-sensitive, and reproducible analytical method for quantification of protein carbonyl, the widely accepted biomarker of protein oxidative damage. Specific objectives of this work are 1) the design and synthesis of a pair of Rhodamine dye derivatives for use as SERS labels of protein carbonyls. 2) the determination of the sensitivity, accuracy, reproducibility, and dynamic range of the SERS based protein carbonyl quantification technique. 3) the experimental and computational investigation of the effect of peptide and protein conjugation on adsorption, aggregation and the SERS activity of the labeling dyes. In addition, the sensitivity, accuracy, and reproducibility of the SERS method will be compared with the two most popular bioanalytical methods: mass spectroscopy and fluorescence. The primary educational goals of the proposal are: 1) to continue engaging and mentoring undergraduate students in cross-disciplinary research in Dr. Zhang's lab from both Mississippi State University (the PI's home university) and Mississippi University for Women, a nearby non-PhD granting institution and 2) to help attract Mississippi high school students into STEM areas by partnering with a nearby high school that has a high drop-out and low college-attendance rate. By participating in the proposed research, high school, undergraduate, and graduate students will gain valuable experience in the area of nanoparticle interfacial interactions and optical spectroscopy. In an effort to enhance chemistry education in the partner high school, The PI and his group will assist the partner high school chemistry teacher in setting-up a teaching laboratory, to design and deliver chemistry experiments. The undergraduate student and high school student researchers in the PI's lab will share their college and research experience with their peers in the partner high school in order to inspire high school students to pursue careers in STEM areas. The learning from this long-term college/high school partnership will be shared nationwide through national conferences and/or journal publication. Dr. Zhang hopes to stimulate more creative ideas to promote education in less developed regions. Progress of the research and educational activities supported by this award will be made public on the PI's research website at http://www.chemistry.msstate.edu/people/faculty/detail.php?faculty=zhang