Fundamental and Mechanistic Studies of Negative Ion Electron Capture Dissociation (niECD)

该奖项由化学测量和成像（CMI）项目颁发，支持密歇根大学的Kristina ha<s:1> kansson教授探索负离子电子捕获解离（niECD）的机制和改进分析效用的工作，niECD是最近在PI实验室发现的一种新型串联质谱技术。本研究的目的是进一步研究niECD的反直觉过程，该过程导致电荷增加而不是其他离子-电子反应中典型的电荷减少。niECD的进一步发展将为重要的低水平酸性生物大分子（如磷酸和硫多肽、完整的酸性蛋白质和rna -小分子复合物）的详细结构表征提供新的手段。这项工作的教育目标包括在跨学科环境中培养研究生，以及参加由密歇根大学女性科学与工程项目提供的K-12暑期项目“科学与工程女孩”。后一种活动的目的是通过发现盒子里的东西不用打开就能看出来，从而提高孩子对科学方法的兴趣。该研究的广泛影响包括引入生物分子结构表征的新技术，对药物发现具有重要意义，并增强了对生物体分子基础的理解。

This award by the Chemical Measurement and Imaging (CMI) program supports work by Professor Kristina Håkansson at the University of Michigan to explore the mechanism and improve the analytical utility of negative ion electron capture dissociation (niECD), a novel tandem mass spectrometry technique recently discovered in the PI's laboratory. The goal of this research is to further examine the counter-intuitive process of niECD, which results in charge-increased rather than the typical charge-decreased species in other ion-electron reactions. Further development of niECD will allow access to novel means for detailed structural characterization of important low-level acidic biomacromolecules such as phospho- and sulfopeptides, intact acidic proteins, and RNA-small molecule complexes. The educational goals of this work include training of graduate students in an interdisciplinary environment as well as participation in a K-12 Summer Program "Girls in Science and Engineering" offered by the University of Michigan's Women in Science and Engineering Program. The latter activities are aimed at boosting an interest in the scientific method at a young age through the discovery that the contents of a box can be elucidated without opening it. The broader impact of the research includes introduction of novel technology for biomolecular structural characterization with important implications for drug discovery and an enhanced understanding of the molecular basis of living organisms.