Magnetic bead based preconcentration and sampling for biosensing in complex media

Hesketh1159726生物分析中的一个关键挑战是从复杂的基质中捕获目标。我们建议开发一种新的样品制备和预富集微流控技术，利用软磁结构和旋转磁场引导的功能化磁珠来混合和收集特定的试剂。将开发一个中尺度计算模式来研究捕获过程的关键特征。长期目标是开发一种实用、紧凑、便携的复杂混合物的预浓缩和病原体净化系统，该系统对食品和环境安全非常重要，可以应用于广泛的检测。智力优势：使用磁珠采样的一个基本限制是目标捕获效率低。磁引导样品预处理微流控系统的最佳操作条件将作为目标相对于微珠的大小、相对流动和微珠速度、目标与微珠之间的粘附力和其他因素的函数来建立。广泛影响：总体目标是演示从与食品安全相关的复杂矩阵现实世界样品中进行有效采样。利用旋转的外部磁场和微尺度的软磁特性，可以操纵磁珠的移动和放置，以提高目标捕获能力。这种新方法将对目标分析物处于低浓度或样品基质复杂的许多类型的生物分析产生重大影响，包括临床分析。跨学科和教育影响：这种基于珠粒的采样和混合的跨学科研究需要机械工程、电气工程和食品科学方面的专业知识。佐治亚大学食品安全中心将展示优化的实验射流装置在食品样品中病原体检测中的应用。这项工作将对许多类型的生物检测产生重大影响，包括临床检测，在这些检测中，目标在复杂基质中的低浓度是复杂的。该项目将涉及和培训研究生和本科生、高中教师和高中生。

Hesketh1159726A key challenge in bioassays is the capturing of a target from a complex matrix. We propose to develop a new sample preparation and pre-concentration microfluidic technique from complex mixtures that uses functionalized magnetic beads guided by soft-magnetic structures and rotating magnetic fields to mix and collect specific reagents. A mesoscale computational model will be developed to study the key characteristics of the capture process. The long term objective is to develop a practical compact portable pre-concentration and pathogen purification system for complex mixtures important for food and environmental safety that can be applied to a wide range of assays.Intellectual Merit: A fundamental limitation of sampling with magnetic beads is low efficiency of target capture. Optimal operation conditions for magnetic-guided sample preconditioning microfluidic systems leading to high capture efficiency will be established as a function of the size of the target relative the bead, the relative flow and bead velocity, adhesion forces between the target and bead and other factors.Broader Impact: The overall objective is to demonstrate efficient sampling from a complex matrix real world sample of relevance to food safety. Utilizing rotating external magnetic fields and microscale soft magnetic features the movement and placement of magnetic beads can be manipulated to improve target capture. This novel method will have significant impact on many types of bioassays where a target analyte is at low concentration or where the sample matrix is complex including clinical assays.Interdisciplinary and Education Impact: This interdisciplinary research on bead based sampling and mixing requires expertise in mechanical engineering, electrical engineering and food science. Application of the optimized experimental fluidic device to the detection of pathogens in food samples at the Center for Food Safety at University of Georgia will be demonstrated. This work will have significant impact on many types of bioassays including clinical assays, where a target is at low concentration in a complex matrix is complex. This project will involve and train graduate and undergraduate students, high school teachers and high school students.