Collaborative Research: Tunable aptasensors for analysis and sorting of living cells

[参考译文]智力价值本提案的目标是开发基于核酸适体的生物传感器。基于适体的生物传感器正在成为传统传感器的可行替代方案，传统传感器采用抗体来识别目标分子。基于适体的生物传感器在化学上和热上更稳定，而且可能是无试剂的——也就是说，不需要额外的处理步骤来产生响应目标分子的信号。我们的建议旨在解决再生这些生物传感器的一个独特挑战，以便使相同的传感器可用于连续在线监测。我们建议使用外加电压在时间和/或空间上调节和控制感应电极的亲和力，以确保一些电极检测关联而其他电极监测解离事件。这样就可以监测目标分子水平的上下变化。更广泛的影响我们提议的目标是开发新的、基于适配体的微系统，用于细胞的分析和分选。我们相信，这种适体传感器在监测活细胞分泌的蛋白质水平的动态变化方面特别有利，在生物技术、细胞/组织工程和传染病检测方面有许多应用。除了生物传感外，适配体修饰的表面还可用于从异质样品（如血液）中分离细胞。本提案将结合基础科学与生物工程的各个方面，通过开发可调谐的适体包覆装置，进一步提高细胞分析和操作的最新水平。我们将积极招募研究生，本科生和高中生参与拟议的实验。

1160262/ RevzinThe Intellectual MeritThe goal of this proposal is to develop biosensors based on nucleic acids called aptamers. Aptamer based biosensors are emerging as viable alternative to traditional sensors that employ antibodies for recognition of target molecules. Aptamer based biosensors are more chemically and thermally stable and may be reagentless - that is no additional processing steps are needed to generate a signal in response to target molecules. Our proposal seeks to addresses a distinct challenge in regenerating these biosensors in order to make the same sensor available for continuous in-line monitoring. We propose to use applied voltage to tune and control affinity of sensing electrodes in temporal and/or spatial manner to ensure that some electrodes are detecting association while others are monitoring dissociation events. This way up and down changes in the level of target molecules may be monitored. Broader ImpactThe goal of our proposal will be to develop novel, aptamer-based microsystems for analysis and sorting of cells. It is our belief that such aptasensors are particularly advantageous for monitoring dynamic changes in the levels of proteins secreted by living cells and have numerous applications in biotechnology, cell/tissue engineering and infectious disease detection. In addition to biosensing, aptamer-modified surfaces may be used for affinity separation of cells from heterogeneous samples such as blood. This proposal will combine aspects of basic science with bioengineering to further enhance state of the art in cell analysis and manipulation by developing tunable aptamer-coated devices. We will actively recruit graduate, undergraduate and high school students to participate in the proposed experiments.