MRI: Acquisition of a Fluorescence Activated Cell Sorter (FACS)

该提案旨在收购最先进的荧光激活细胞分选仪（FACS），该分选仪将成为流式细胞术/细胞分选共用设施的一部分，吸引诺曼俄克拉荷马州大学（University of Oklahoma）校园内的各种研究项目。细胞分选被认为是从生命科学中的基础生物学到转化生物医学工程研究的重要和不可或缺的工具。在OU-Norman校区获得最先进的细胞分选能力不仅可以提供所需的资源，还可以为OU-Norman校区现有的细胞表征设施提供有力的补充：扫描电子显微镜，多光子激光扫描共聚焦显微镜和原子力显微镜。将这些显微镜技术提供的有关单个细胞结构的详细信息与（i）高通量流式细胞术提供的有关细胞群的广泛统计信息相结合，以及（ii）通过免疫表型识别和分选复杂群体细胞的能力将促进整个校园的新的和创新的研究方向。

This proposal is for the acquisition of a state-of-the-art Fluorescence Activated Cell Sorter (FACS) that will become part of a flow cytometry/cell sorting shared use facility appealing to a diverse set of research programs on the University of Oklahoma (OU) campus in Norman. Cell sorting is recognized as important and indispensable tools in fields ranging from basic biology in the life sciences to translational biomedical engineering research. Acquisition of state-of-the-art cell sorting capabilities on the OU-Norman campus would not only make available needed resources, but would also provide a powerful complement to existing cellular characterization facilities on the OU-Norman Campus: Scanning Electron Microscopy, Multi-photon Laser Scanning Confocal Microscopy, and Atomic Force Microscopy. Combining detailed information about individual cell structures provided by these microscopy techniques with (i) the extensive statistical information about cell populations from high throughput flow cytometry and (ii) the ability to identify and sort cells from complex populations by immunophenotyping will foster new and innovative research directions across the OU campus.