MRI: Development of a Stimulated Scattering Microscope for Cell Imaging

本研究的目的是开发一种新型的基于受激瑞利/布里渊散射的非线性散射显微镜，它将能够测量细胞的热和弹性性质。这项工作的核心部分是组装一种新的激光系统，该系统将提供非常快速的调谐和出色的噪声特性。在这项工作中，SRI将(1)组装激光系统，(2)将激光系统与其相关的电子设备结合起来，(3)通过细胞成像来评估仪器的性能和操作。这项工作将为生物学和医学研究带来一种新型的非线性显微镜，它将补充现有的非线性显微镜技术，包括使用荧光的多光子显微镜、二次谐波和三次谐波产生、相干反斯托克斯拉曼(CARS)显微镜和四波混频。SRI最近在受激瑞利/布里渊散射方面取得了新的进展，这将使细胞能够快速成像，而不会对细胞造成损害。SRI计划开发的显微镜将允许以新的方式观察通过机械力、细胞运动、细胞分裂和其他涉及细胞粘弹性特性变化的方面进行的信号转导。

ABSTRACTThe objective of the proposed study is to develop a new type of nonlinear scattering microscopy based on stimulated Rayleigh/Brillouin scattering that will enable measurement of thermal and elastic properties of cells. A central part of this work is assembly of a novel laser system that will provide very rapid tuning and excellent noise properties. In this effort SRI will (1) assemble the laser system, (2) combine the laser system with its associated electronics, and (3) evaluate instrument performance and operation through imaging of cells. This work will bring a new type of nonlinear microscopy to biology and medical research that will complement existing nonlinear microscopy techniques, including multiphoton microscopy using fluorescence, second-harmonic and third harmonic generation, coherent anti-Stokes Raman (CARS) microscopy, and four-wave mixing. SRI has recently developed new advances in stimulated Rayleigh/Brillouin scattering that will enable rapid imaging of cells without causing damage to the cells. The microscope SRI proposes to develop will allow a new look at effects such as signal transduction through mechanical forces, cell motility, cell division, and other aspects involving changes in cellular viscoelastic properties.