Nanoscaled Architectures for highly Sensitive Biosensing of Small molecules

结合先进的生物分子技术，化学和纳米技术，该项目旨在研制超灵敏和特异性免疫传感器，用于检测复杂环境中的优先污染物。它依赖于四个相互依存的任务：1）生产和详细表征针对多环芳烃（苯并[a]芘），药物（双氯芬酸）和藻类毒素（微囊藻毒素）的高亲和力单克隆抗体。2)纳米结构化的表面，作为适当的平台，结合分子探针。3)使用金纳米颗粒及其优化设计，以增强光学检测技术（表面等离子体共振、SPR、表面增强拉曼散射、Sers和化学发光读数）的灵敏度。4)测试开发使用石英晶体微天平与耗散测量（QCM-D）以及SPR和Sers，以达到上述污染物在低ng/L范围内的检测限。同时，将开发一种新的基于微阵列的自动化流通ELISA。所有测试都将使用真实的样品和LC-MS进行验证。该项目的成功依赖于相关合作伙伴、生物化学家、分子化学家、表面科学家的互补专业知识以及他们之间的不断交流。合作伙伴2将为其他人提供新的仿射抗体，并将开发多分析物测试。合作伙伴1将专注于表面纳米结构化以及SPR，Sers和QCM的所有检测测量。合作伙伴3将设计和合成新的分子结构（官能化聚氧乙烯醚），产生一个非常原始的表面化学图案化的方式。这个项目雄心勃勃，可能有风险，因为每一步都有新的发展;由于已经取得了初步成果，它也很有希望。预计在基本和应用层面都将取得成果。毫无疑问，在高影响力的科学期刊上发表论文是可以期待的。目标技术在与健康和环境保护有关的各种应用领域也会引起相当大的兴趣。

Combining advanced biomolecular technology, chemistry and nanotechnology, this project aims at the elaboration of ultra-sensitive and specific immunosensors for the detection of priority pollutants in complex environments. It relies on four interdependent tasks: 1) Production and detailed characterization of high-affine monoclonal antibodies towards polycyclic aromatic hydrocarbons (benzo[a]pyrene), pharmaceuticals (diclofenac) and algae toxins (microcystins). 2) Nanostructuring of surfaces to serve as appropriate platforms for binding molecular probes. 3) Use of gold nanoparticles, and their optimized design, to enhance the sensitivity of optical detection techniques (Surface Plasmon Resonance, SPR, Surface Enhanced Raman Scattering, SERS, and chemiluminescence readout). 4) Test development using Quartz Cristal Microbalance with dissipation measurements (QCM-D) as well as SPR and SERS with the objective of reaching detection limits in the low ng/L-range for the above cited pollutants. In parallel, a new automated microarray-based flow-through ELISA will be developed. All tests will be validated using real samples and LC-MS. The success of this project relies on the complementary expertise of the involved partners, biochemists, molecular chemists, surface scientists, and on constant exchanges between them. Partner 2 will provide others with new affine antibodies, and will develop the multi-analyte test. Partner 1 will focus on surface nanostructuration as well as on all the detection measurements by SPR, SERS and QCM. Partner 3 will design and synthesize new molecular architectures (functionalized polyoxometalates), yielding a very original way of surface chemical patterning. This project is ambitious and likely risky in the sense that each step includes new developments; it is also very promising thanks to already obtained preliminary results. Results are expected both at fundamental and applied levels. Publications in high impact scientific journals can be undoubtedly expected. The aimed technology would also be of considerable interest in various applied domains linked to health and environmental protection.