Modulation of ABA signaling by Ca2+-dependent posttranslational regulation

该项目旨在研究调节SNF 1相关激酶2（SNRK 2）响应干旱激素脱落酸（阿坝）激活的复杂调控网络，并确定SNRK 2靶蛋白。总的来说，该项目的重点是磷酸化事件和蛋白质-蛋白质相互作用，以及与通过SNRK 2激酶严格控制信号传导的其他植物信号传导途径的相互作用。具体而言，该项目旨在：（a）分析SNRK 2激酶通过上游事件的调节，包括钙调神经磷酸酶B样（CBL）蛋白和CBL相互作用蛋白激酶（CIPK）活性，茉莉酸信号传导和细菌效应物，（B）在蛋白质组水平上表征ABA诱导的磷酸化事件，并通过比较激酶突变体和野生型植物中的磷酸化模式来鉴定SNRK 2激酶底物。本项目将深入了解阿坝与其他激素和病原菌激活的信号通路在气孔调节中的复杂相互作用。此外，它将提供一个新的途径，非生物信号事件的动力学分析，旨在确定病原菌控制气孔调节事件的分子基础。

The proposed project aims to investigate the complex regulatory network that modulates activation of SNF1-RELATED KINASES 2 (SNRK2) in response to the drought hormone Abscisic acid (ABA) and to identify SNRK2 target proteins. In general, this project is focused on phosphorylation events and protein-protein interactions as well as interaction with other plant signaling pathways that tightly control signaling through SNRK2 kinases. Specifically this project aims to: (a) analyze the regulation of SNRK2 kinases via upstream events, including CALCINEURIN B LIKE (CBL) proteins and CBL-INTERACTING PROTEIN KINASE (CIPK) activity, Jasmonic acid signaling and bacterial effectors, (b) characterize ABA-induced phosphorylation events on a proteome level and identify SNRK2 kinase substrates by comparison of phosphorylation patterns in kinase mutants and wildtype plants. This project will generate insight into the complex interactions between ABA and other hormone and pathogen-activated signaling pathways in stomatal regulation. Moreover, it will provide a new approach to kinetic analysis of abiotic signaling events and aims to identify the molecular basis of pathogen control of stomatal regulation events.