SBIR Phase II: A Surface Acoustic Wave Based Ion Source

这个小企业创新研究（SBIR）第二阶段项目将开发一个高-？]吞吐量--？]声表面波雾化（HTP）SAWN）离子源。在第一阶段开发的SAWN离子源产生了一种设备，该设备在两种最流行的MS电离技术之间具有竞争性性能：电喷雾电离（ESI）和基质-？]辅助激光解吸电离（MALDI）。SAWN提供了从像MALDI那样的平面表面电离的便利性，但是ESI在大气压下的性能特征（Heron等人，Anal. 2010）。然而，在第一阶段结束时，SAWN仍然是低吞吐量，并在技术上处于早期发展阶段。第二阶段的建议将完善SAWN的性能，将其耦合到一个新的MS接口，有效地样品溶剂化分析物产生的SAWN使其立即提供所有主要制造商？金融服务工具。第二阶段将导致1）通过研究锂酸盐以外的选项优化压电晶片; 2）自动化，机器人，HTP--？] SAWN将使现有ESI的吞吐量增加一倍？]基于分析;以及3）在阶段I中开发的制造更经济且操作更有效的同心环叉指换能器电极设计。该项目更广泛的影响/商业潜力将提供高--？]吞吐量--？]声表面波雾化（HTP）SAWN）离子源，提供了易用性;较低的离子能量和能力，即时监测化学反应。SAWN通过直接比较（Huang et al. JASMS 2012; Yoon et al. Anal Chem 2012）产生的离子能量低于ESI和MALDI推断。除此之外，HTP？SAWN，第二阶段将导致SAWN与所有主要制造商兼容？金融服务工具。SAWN还将与真实的时间直接分析（DART）相结合，以扩展SAWN和DART的能力;例如，该组合将提供从SAWN芯片解吸到DART流中的乳剂中的化合物的二次电离。此外，通过HTP进行的干血斑分析--？] SAWN将使用溶酶体贮积症的新生儿错误测定法作为示例进行开发。本试验将用于证明SAWN用于反应产物即时分析的潜力。SAWN具有更广泛的潜力，可以直接分析任何数量的化学或生物化学反应，这些反应在几秒钟内通过监测细胞分泌物或细菌通讯进行。

This Small Business Innovation Research (SBIR) Phase II project will develop a high--?]throughput --?] surface acoustic wave nebulization (HTP--?]SAWN) ion source for mass spectrometry (MS). The SAWN ion source developed during phase I resulted in a device that has competitive performance between the two most popular ionization techniques for MS: electrospray ionization (ESI) and matrix--?]assisted laser desorption ionization (MALDI). SAWN offers the convenience of ionization from a planar surface like MALDI, but the performance characteristics of ESI at atmospheric pressure (Heron et al. Anal. Chem. 2010). However, at the end of phase I SAWN remains low throughput and at an early developmental stage technologically. The phase II proposal will refine SAWN performance by coupling it to a novel MS interface that efficiently samples solvated analytes produced by SAWN making it immediately available on all major manufacturer?fs instruments. Phase II will result in an 1) optimized piezoelectric wafer by investigating options other than lithium niobate; 2) automated, robotic, HTP--?]SAWN that will double the throughput of an existing ESI--?]based assay; and 3) a more economical to manufacture and efficient to operate concentric ring interdigitated transducer electrode design developed in phase I. The broader impact/commercial potential of this project will provide a high--?]throughput --?] surface acoustic wave nebulization (HTP--?]SAWN) ion source for mass spectrometry that offers ease of use; lower ion energetics and the capability to instantaneously monitor chemical reactions. SAWN has been shown by direct comparison to generate ions of lower energy (Huang et al. JASMS 2012; Yoon et al. Anal Chem 2012) than ESI and by inference MALDI. In addition to HTP--?]SAWN, phase II will result in SAWN being compatible with all major manufacturer?fs instruments. SAWN will also be combined with direct analysis in real time (DART) to expand the capability of SAWN and DART; e.g. the combination will provide secondary ionization of compounds in emulsions desorbed from the SAWN chip into the DART stream. Additionally, dried blood spot analysis by HTP--?]SAWN will be developed using a newborn error assay for lysosomal storage disorders as an example. This assay will be used to demonstrate the potential of SAWN for immediate analysis of reaction products. SAWN has the broader potential for direct analysis any number of chemical or biochemical reactions within seconds of them having been carried out from monitoring cellular secretions or bacterial communication.