CSBR: Living Stocks: Species validation, enhanced data access and remote archiving of the Phaff Yeast Culture Collection

加州大学戴维斯分校的Phaff酵母菌培养收藏对酵母生理学、酵母生态学（特别是酵母与昆虫的关联）、比较基因组学和包括发现新酵母菌种在内的分类学等基础研究特别有用。Phaff收集的酵母还用于应用研究，如食品和饮料发酵、食品变质、生物燃料探索，以及开发鉴定引起人类感染的酵母的改进方法。通过本项目支持的活动，本科生将获得分类学和分子生物学方面的培训。此外，包括代表性不足群体在内的中学生将通过加州大学戴维斯分校生物训练营的演讲学习微生物学和文化收藏。法夫酵母菌培养收藏是世界上第四大酵母菌收藏。它包含7000多个菌株，属于大约800种酵母。大多数菌株是独特的，无法从任何其他来源获得。该项目提出了一个重大的改革收集，使其更有用，可见，和有价值的全球酵母研究和教育界。这个项目将使Phaff系列与欧洲和亚洲的同类系列保持一致。目标包括：1)通过DNA测序验证和更新酵母物种ID； 2)升级到更强大的数据库、网站和在线菌株目录；3)通过接入全球微生物目录、GenBank和MycoBank扩大全球可访问性；4)在远程低温保存设施完成酵母档案存储。通过在GenBank中发布DNA序列并扩展Phaff馆藏在线目录上的信息，该馆藏将对所有用户更加可见和有价值。通过该项目获得的经验教训将通过美国国家科学基金会资助的美国文化收藏网络与其他美国文化收藏策展人分享。更多信息请访问：http://phaffcollection.ucdavis.edu。

The Phaff Yeast Culture Collection, at the University of California Davis, is particularly useful for basic research such as yeast physiology, yeast ecology (especially yeast-insect associations), comparative genomics, and taxonomy including discovery of new yeast species. The Phaff collection of yeasts are also used for applied research such as food and beverage fermentation, food spoilage, biofuels exploration, and the development of improved methods to identify yeasts that cause human infections. Through the activities supported in this project undergraduate students will receive training in taxonomy and molecular biology. In addition, middle school students including underrepresented groups will learn about microbiology and culture collections through presentations in the UC Davis Bio Boot Camp. The Phaff Yeast Culture Collection is the fourth largest yeast collection in the world. It contains over 7,000 strains belonging to approximately 800 yeast species. Most strains are unique and not available from any other source. This project proposes a major overhaul of the collection to make it more useful, visible, and valuable to the worldwide yeast research and education community. This project will align the Phaff collection with comparable collections in Europe and Asia. Aims include: 1) Validate and update yeast species ID by DNA sequencing, 2) Upgrade to a more powerful database, website and online strain catalog, 3) Expand global accessibility by plugging into the Global Catalog of Microorganisms, GenBank and MycoBank, and 4) Complete the archival storage of yeasts at a remote cryopreservation facility. By posting DNA sequences of accessions in GenBank and expanding information available on the Phaff collection online catalog the collection will be more visible and valuable for all users. Lessons learned through this project will be shared with other US culture collection curators through the NSF-funded US Culture Collection Network. More information is publically available at: http://phaffcollection.ucdavis.edu.