CAREER: Artificial Cell Membranes for Ultra-Pure, High Throughput Cellular Isolation

CBET-1351531基于BerronCell的疗法，即细胞被移植以自然恢复生物功能，代表着令人兴奋的未来生物医学应用。关键的是，目前的高纯度细胞分选技术都是耗时的过程，缺乏速度来满足研究中新出现的对纯化细胞的广泛需求。PI最近发现了一种快速分离细胞的方法，在这种方法中，批次大小几乎不受限制，不能检测到非特异性细胞。这项名为抗原特异性裂解(ASL)的技术有选择地将目标细胞包裹在临时保护膜中，然后摧毁所有未被包裹的细胞，以产生活的、纯化的细胞。这项拟议的工作将研究在细胞分离中管理ASL的基本机制和关系。这一职业发展计划为开发ASL的长期研究计划提供了基础，ASL用于高速分离稀有生物制品，从低表达标记中产生高纯度的分离。这项研究探索了细胞保护的基本问题，并将这些发现应用于细胞分离技术，该技术有可能将非靶向细胞的存在减少到无法检测的水平，同时以极高的分选率进行分选。生物分离将在美国经济的未来发挥关键作用，但许多大专院校缺乏足够的人员配备和专业知识来提供这些课程。将开发一个远程学习生物分离课程，以接触到地理位置不同的机构，使之能够向数百英里外的学生提供强大的生物分离材料。本课程将作为一个平台，探讨协作学习技术在远程学习环境中的局限性。生物分离教育的一个重大限制是缺乏在线学习工具。ASL建立在不同的生物分离原理(如裂解、过滤、沉淀、吸附)的基础上，这项职业发展计划将ASL作为在线生物分离截屏视频-教育短片的核心。这些视频在学生中很受欢迎，但目前仅限于化学工程核心课程。开发的截屏视频将与NSF资助的LearnChemE.com网站合作传播，在过去12个月中，现有的截屏视频在该网站上的浏览量已达160万次。此外，LearnChemE.com团队将对截屏视频进行评论和编辑，以确保最高质量的教育产品。

CBET-1351531BerronCell-based therapies, where cells are transplanted to naturally restore biological function, represent an exciting future biomedical application. Critically, current techniques for high-purity cell sorting are time-intensive processes which lack the speed to satisfy the emerging widespread need for purified cells in research. The PI recently discovered a rapid cell isolation method in which batch size is virtually unlimited and nonspecific cells are undetected. The developed technology, Antigen Specific Lysis (ASL), selectively coats targeted cells in a temporary protective membrane, then destroys all uncoated cells to yield viable, purified cells. The proposed work will investigate fundamental mechanisms and relationships governing ASL in cellular isolation. This career development plan provides the foundation for a long term research program in the development ASL for the high speed isolation of rare biological products which yields a high-purity isolate from poorly-expressed markers. This research explores fundamental questions of cellular protection, and applies these findings to a cellular isolation technique which has the potential to reduce the presence of non-targeted cells to undetectable levels while sorting at exceptionally high rates. Bioseparations will play a critical role in the future of the US economy, yet many postsecondary institutions lack sufficient staffing and expertise to offer these courses. A distance learning Bioseparations course will be developed to reach geographically disparate institutions, enabling a robust offering of Bioseparations materials to students several hundreds of miles away. This course will serve as a platform to probe the limits of collaborative learning techniques in distance learning environments. A significant limitation to Bioseparations education is a lack of online learning tools. ASL is built upon a foundation of diverse Bioseparations principles (e.g. lysis, filtration, sedimentation, adsorption), and this career development plan uses ASL as the centerpiece to online Bioseparations screencasts - short educational videos. These videos are popular among students, yet are currently limited in Chemical Engineering to core courses. The developed screencasts will be disseminated in collaboration with the NSF-funded, LearnChemE.com site, where the existing screencasts have had 1.6 million views in the last 12 months. Further, the screencasts will be critiqued and edited by the LearnChemE.com team, ensuring the highest quality educational products.