IDBR TYPE A: Development of a Line Confocal Bessel Beam Platform for High-speed High-Volume 3D Imaging In Vivo

美国国家科学基金会IDBR奖授予加州大学洛杉矶分校的Katsushi Arisaka教授及其合作者，他们开发了贝塞尔光束线共聚焦显微镜。该项目的目标是开发一种能够实时记录体内三维细胞结构的系统，具有特殊的穿透深度，并且对目标样品的损害最小。该系统将通过促进细胞观察和系统级生物分析来促进科学理解。值得注意的是，该系统将在一个允许观察发育现象的时间范围内，以超分辨率实现前所未有的细胞动力学高速记录。更广泛的影响是一个具有成本效益，易于配置和用户友好的3D成像系统，用于科学家在几天的时间范围内对动态生物样品进行体内结构表征。此次合作将产生一种可用且可复制的显微镜，该显微镜将显著推进有关胚胎发育、动态神经网络功能、细胞分化和调控的可获得信息，同时实现大容量3D细胞成像。该项目通过将显微镜的发展融入加州大学洛杉矶分校的Arisaka博士开发的一门新颖的跨学科实验课程，直接将教育和研究目标结合起来。因此，系统的开发和建设将成为直接促进学生学习的教育平台。此外，该系统将被安置在加州纳米系统研究所（CNSI）的先进光学显微镜设施中，从而广泛地为扩展的科学界提供可用性。该奖项是由两个项目联合颁发的，(1)生物基础设施部（生物科学理事会）的生物研究仪器开发，以及(2)化学、生物工程、环境和运输系统部（工程理事会）的生物医学工程。

This NSF IDBR award is made to Prof. Katsushi Arisaka and collaborators at the University of California, Los Angeles, to develop a Bessel beam line confocal microscope. The goal of this project is to develop a system enabling the recording of three-dimensional cell structure in vivo, in real-time, with exceptional penetrating depth, and with minimal damage to the targeted sample. The proposed system will advance scientific understanding by facilitating cellular observation and systems level biologic analysis. Significantly, this system will enable the unprecedented high-speed recording of cell dynamics at super-resolution, in a temporal range permitting observation of developmental phenomena.The broader impact is a cost-effective, easily configurable and user-friendly 3D imaging system for use by scientists towards the in vivo structural characterization of dynamic biological samples over a timespan of days. This collaboration will generate an available and reproducible microscope that significantly advances obtainable information concerning embryo development, dynamic neural network function, cellular differentiation and regulation, while enabling high-volume 3D cell imaging. This project directly integrates educational and research goals through incorporation of microscope development into a novel, interdisciplinary laboratory course developed by Dr. Arisaka at UCLA. Thus, development and construction of the system will serve as an educational platform directly fostering student learning. Moreover, the system will be housed in the Advanced Light Microscope Facility at the California NanoSystems Institute (CNSI), resulting in widespread availability to the extended scientific community.This award is being made jointly by two Programs- (1) Instrument Development for Biological Research, in the Division of Biological Infrastructure (Biological Sciences Directorate), and (2) Biomedical Engineering, in the Division of Chemical, Bioengineering, Environmental and Transport Systems (Engineering Directorate).