IDBR: TYPE A: Auto-CATM Development: An Instrument for Dynamical Fingerprinting

IDBR：TYPE A：Auto-CATM 开发：动态指纹识别工具

• 批准号: 1556359
• 负责人: Andrea Markelz
• 金额: $ 39.55万
• 依托单位: SUNY at Buffalo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2020-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1556359&HistoricalAwards=false
• 关键词: IDBR; TYPE; Auto; CATM; Development

An award is made to the State University of New York at Buffalo to develop an instrument to measure the internal vibrations of proteins. Proteins are large molecules with thousands of atoms and perform most cellular functions. Proteins consist of long chains of amino acids, and these chains fold into specific structures. In general, proteins must alter their shape in order to perform their biological function. For example, Hemoglobin, the protein that transports oxygen, changes shape in order for oxygen to bind. The internal vibrations of the protein structure that enable this change in shape are critical for understanding protein function. Recently a method has been devised to measure protein internal vibrations, called crystal anisotropic terahertz microscopy (CATM). The instrumentation and protocol development in this project makes CATM accessible to non-specialists, and enables broad application of the technique. The development of this instrument leads to the interdisciplinary education of two graduate students and two undergraduates. Integrated with the instrument development are educational and outreach activities that include an undergraduate seminar course and a week-long summer program for 7-12th grade inner city students from underrepresented groups. The goal of this project is to produce a compact turn-key spectroscopic system for the routine characterization of intramolecular vibrations of proteins. The technique called crystal anisotropic terahertz (THz) spectroscopy (CATM) uniquely measures these motions. Auto-CATM provides non-experts with the ability to quickly and easily categorize and classify biomacromolecules. The mini-system from Zomega Terahertz Corp. is the starting platform for the final instrument. A proof-of-concept database, and molecular modeling and data visualization software toolbox facilitate the development this new instrument. This instrument provides insights into how intramolecular vibrations are impacted by frequently modified parameters such as binding state and temperature. The technology developed for Auto-CATM also enable new rapid measurements for both bulk and micron scale materials and thus impact solid state physics and materials science.

纽约州立大学布法罗分校（State University of New York at Buffalo）因开发出一种测量蛋白质内部振动的仪器而获奖。蛋白质是由数千个原子组成的大分子，执行大多数细胞功能。蛋白质由长链氨基酸组成，这些氨基酸链折叠成特定的结构。一般来说，蛋白质必须改变其形状才能发挥其生物学功能。例如，血红蛋白，一种运输氧气的蛋白质，为了使氧气结合而改变形状。使这种形状变化的蛋白质结构的内部振动对于理解蛋白质的功能至关重要。最近发明了一种测量蛋白质内部振动的方法，称为晶体各向异性太赫兹显微镜（CATM）。本项目的仪器和协议开发使非专业人员可以访问CATM，并使该技术得到广泛应用。该仪器的发展导致了两名研究生和两名本科生的跨学科教育。与仪器开发相结合的是教育和推广活动，包括本科研讨会课程和为期一周的暑期课程，面向来自代表性不足群体的7-12年级市中心学生。该项目的目标是生产一个紧凑的交钥匙光谱系统，用于蛋白质分子内振动的常规表征。这种被称为晶体各向异性太赫兹（THz）光谱（CATM）的技术可以独特地测量这些运动。Auto-CATM为非专家提供了快速方便地对生物大分子进行分类和分类的能力。来自Zomega太赫兹公司的迷你系统是最终仪器的启动平台。一个概念验证数据库，分子建模和数据可视化软件工具箱促进了这种新仪器的开发。该仪器可以深入了解分子内振动如何受到频繁修改的参数（如结合状态和温度）的影响。为Auto-CATM开发的技术还可以对块状和微米级材料进行新的快速测量，从而影响固态物理和材料科学。

项目成果

Moving in the Right Direction: Protein Vibrational Steering Function

• DOI: 10.1016/j.bpj.2016.12.049
• 发表时间: 2017-03-14
• 期刊: BIOPHYSICAL JOURNAL
• 影响因子: 3.4
• 作者: Niessen, Katherine A.;Xu, Mengyang;Markelz, Andrea G.
• 通讯作者: Markelz, Andrea G.

Modulated orientation-sensitive terahertz spectroscopy

调制定向敏感太赫兹光谱

• DOI: 10.1364/prj.4.0000a1
• 发表时间: 2016
• 期刊: Photonics Research
• 影响因子: 7.6
• 作者: Singh, Rohit;George, Deepu Koshy;Bae, Chejin;Niessen, K. A.;Markelz, A. G.
• 通讯作者: Markelz, A. G.