Deep Biosphere Quantification in Chesapeake Bay Impact Structure Sediments

该项目将调查切萨皮克湾撞击结构撞击后沉积物的深层生物圈。主要目的是活体原核生物的可视化和定量以及丰富的原核生物群的定量。利用定量PCR和CARD - FISH技术，在太平洋深海沉积物中发现了相当比例的原核细胞（AODC）是活菌。我们计划利用这些技术将这一重要发现外推到切萨皮克湾撞击结构沉积物中。定量PCR也将用于从丰富的16S rDNA簇和功能基因（如硫酸盐还原原核生物的异化亚硫酸盐还原酶基因（dsrAB））中定量古细菌和细菌。此外，烃类降解微生物将被富集、分离和描述。将对50个不同深度的沉积物岩心样本进行分析，以探索“深层生物圈”。

The project will investigate the Deep Biosphere of the Chesapeake Bay Impact Structure post-impact sediments. The main objective is the visualization and quantification of living prokaryotes and the quantification of abundant prokaryotic groups. A high proportion of the total number of prokaryotic cells (AODC) has been identified as living Bacteria in deep sediments from the Pacific using quantitative PCR and CARD - FISH. We plan to use these techniques to allow the extrapolation of this important finding to the Chesapeake Bay impact structure sediments. Quantitative PCR will also be used to quantify Archaea and Bacteria from abundant clusters of 16S rDNA and functional genes, e.g. the dissimilatory sulfite reductase gene (dsrAB) of sulfate reducing prokaryotes. In addition, hydrocarbon-degrading microorganisms will be enriched, isolated and described. 50 sediment core samples from various depths will be analyzed to explore the "Deep Biosphere".