OP: Collaborative Research: Multimodal Molecular Spectroscopy and Imaging in Biological Tissue and Historical Artwork

在化学系化学测量和成像计划、物理系原子、分子和光学实验物理计划以及国际科学与工程办公室的支持下，Martin Fischer、Warren Warren、Khanh Kieu教授和他们的团队将开发光学显微镜技术，以创建具有新的分子和结构对比度的高分辨率图像。这些成像方法甚至可以在表面下提供详细的化学信息，并且不需要物理移除样本来创建三维地图。这些技术非常适合生物组织和历史艺术品的成像，在这些地方，样品的移除是受限的，甚至是不可能的。从这些化学图谱中获得的信息可以为开发新的方法来诊断生物组织中的疾病奠定科学基础，并为文物保护者提供修复甚至防止不可替代的艺术品损坏或降解所需的信息。生物组织和历史艺术品中功能的丧失，如绘画中的变色、裂缝或组织中的疾病，通常反映在微观尺度上的分子组成或结构的变化上。诊断评估中的一个主要挑战是，为了获得这些信息，通常需要机械去除样本。为了应对这一挑战，本研究试图开发一种基于非线性泵浦-探测显微镜的多模式检测方法，以高空间分辨率在三维非侵入性地绘制颜料及其周围环境的图谱。具体地说，正在开发的技术获得了新的对比机制(如非线性相位对比)，这些机制提供了更高的特异性和更广泛的内源性靶点。此外，正在研究组织和艺术品中的关键降解过程，包括固有的(例如自然降解)和诱导(例如光降解)过程。最后，该团队正致力于利用这些技术向生物医学和保护科学界传播合适的仪器。

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, the Atomic, Molecular, and Optical Experimental Physics Program in the Division of Physics, and the Office of International Science and Engineering, Professors Martin Fischer, Warren Warren, Khanh Kieu and their groups will develop optical microscopy techniques to create high-resolution images with novel molecular and structural contrast. These imaging methods can provide detailed chemical information even below the surface and do not require the physical removal of samples to create three-dimensional maps. These techniques are ideally suited for imaging of biological tissue and historical works of art, where the removal of a sample is restricted or even impossible. Information obtained from these chemical maps can help lay the scientific foundation for the development of new methods to diagnose diseases in biological tissue and give conservators information needed to repair or even prevent damage or degradation in irreplaceable works of art.The loss of function in biological tissue and historical artworks, such as discoloration or cracking in a painting or disease in tissue, generally is reflected in changes in the molecular composition or structure on a microscopic scale. A major challenge in diagnostic evaluation is that to obtain this information, mechanical sample removal is generally required. In response to this challenge, this research seeks to develop a multimodal detection method based on nonlinear pump-probe microscopy to noninvasively map pigments and their surroundings with high spatial resolution in three dimensions. Specifically, the techniques being developed access novel contrast mechanisms (such as nonlinear phase contrast) which give much higher specificity and a wider range of endogenous targets. Furthermore, critical degradation processes are being studied in tissue and artwork, including inherent (e.g. natural degradation) and induced (e.g. photodegradation) processes. Lastly, the team is working toward dissemination of suitable instruments using these techniques to the biomedical and conservation science communities.