MRI: Acquisition of an ultra performance liquid chromatography mass spectrometer (UPLC-MS) for multi-disciplinary research

该奖项由主要研究仪器(MRI)和化学研究仪器(CRIF)项目支持。加州州立大学富勒顿分校的保拉·哈德森教授及其同事马德琳·拉什、苏达山·库尔瓦德卡尔、尼古拉斯·萨尔扎梅达和亚历山德拉·奥查德获得了一台台式四极液相色谱串联质谱仪(Q-LCMS/MS)。通常，质谱仪是用于识别和表征复杂样品中的少量化学物种的关键分析方法之一。在一个典型的实验中，这些成分流入质谱仪，在那里它们被电离成离子，并测量离子的质量。这种高度灵敏的技术使研究复杂混合物中的分子结构成为可能。带有液体色谱仪的仪器可以在混合物到达质谱计之前将其分离。此次收购加强了该大学和该地区的研究基础设施。该仪器通过让不同的学生群体参与研究和使用这种现代分析技术进行研究培训，扩大了参与度。它还为这所拉美裔服务机构的许多本科生提供培训机会，因为他们是CSUF研究实验室的主要劳动力。此外，CSUF校园的研究项目通过STEM教育区域联盟(项目RAISE)支持的为期10周和12周的暑期研究体验吸引来自当地社区学院的学生。质谱仪加强了各级的研究和教育。该仪器特别影响对大气和环境相关分子的识别和量化的研究。它用于快速、高精度地表征与人类健康和疾病相关的酶的蛋白质-蛋白质相互作用的抑制物。该光谱仪还可用于设计酶分析方法，以鉴定小分子蛋白水解酶抑制剂，并量化用于酶催化剂发现和动力学表征的小分子。

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation (CRIF) Programs. Professor Paula Hudson from California State University Fullerton and colleagues Madeline Rasche, Sudarshan Kurwadkar, Nicholas Salzameda and Alexandra Orchard have acquired a benchtop quadrupole, liquid chromatograph tandem mass spectrometer (Q-LCMS/MS.) In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex samples. In a typical experiment, the components flow into a mass spectrometer where they are ionized into ions and the ions' masses are measured. This highly sensitive technique allows the structure of molecules in a complex mixtures to be studied. An instrument with a liquid chromatograph can separate mixtures of compounds before they reach the mass spectrometer. The acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse groups of students in research and research training using this modern analytical technique. It also provides training opportunities to many undergraduate students at this Hispanic-serving institution since they are the major workforce in research laboratories at CSUF. Additionally, research programs on the CSUF campus engage students from local community colleges through ten and twelve-week summer research experiences supported through the Regional Alliance in STEM Education (Project RAISE).The mass spectrometer enhances research and education at all levels. This instrument especially impacts research on identification and quantification of atmospherically and environmentally relevant molecules. It is used in rapidly characterizing ,with high accuracy, inhibitors of protein-protein interactions of enzymes related to human health and disease. The spectrometer is also used in designing enzyme assays to identify small molecule protease inhibitors and quantify small molecules used in the discovery and kinetic characterization of enzyme catalysts.