MRI: Acquisition of a Hybrid Supercritical Chromatography, High-Pressure Chromatograph, Quadrupole-Time-of-Flight Mass Spectrometer (SFC/UHPLC Q-ToF/MS)

该奖项由主要研究仪器和化学研究仪器计划支持。 来自加州大学滨江的Joseph Genereux教授和同事Ming Tang获得了一种混合超临界色谱、超高压液相色谱、四极杆飞行时间质谱仪（SFC/UHPLC Q-ToF/MS）。一般来说，质谱（MS）是用于识别和表征复杂基质中少量化学物质的关键分析方法之一。在典型的实验中，组分流入质谱仪，在那里它们被电离并测量离子质量。这种高灵敏度的技术可以检测和确定复杂混合物中分子的结构。具有超临界色谱仪的仪器通过在化合物到达质谱仪之前使用高压和高温分离化合物的混合物来提供额外的结构鉴定能力。在ToF方法中，离子的质荷比通过时间测量的方式来确定，其中离子被已知强度的电场加速。此次收购加强了大学和地区的研究基础设施。该仪器通过利用这一现代分析技术让不同的学生参与研究和研究培训，扩大了参与。该仪器作为一种工具，扩大妇女和代表性不足的少数民族在科学和工程研究中的参与，在这个西班牙裔服务机构。这一独特的质谱仪的奖项，旨在加强各级研究和教育。它特别允许使用亲电子足迹来识别复杂介质中的蛋白质构象变化，以及创建用于能量传输的分支树枝状结构。质谱仪有助于表征蛋白质与结构化或修饰的核酸之间的非共价相互作用，并鉴定老化蛋白质组中的天冬氨酸差向异构化。该仪器还用于研究自分选，超分子催化和分子动力学以及研究丝氨酸肽组装。该质谱仪还为研究色氨酸合成酶的结构和机理以及探索具有弱配位碳硼烷阴离子的配体的烯烃聚合催化剂的研究人员提供服务。该仪器还用于分析蛋白质生物合成和蛋白质质量，使用代谢组学破译t细胞异质性，使用不对称方法和合成策略研究神经保护性柠檬苦素。它也被用于研究转录后和翻译后修饰对真核生物翻译起始动力学的影响，并从金（III）络合物中获得实体选择性。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Professor Joseph Genereux from University of California Riverside and colleague Ming Tang have acquired a hybrid supercritical chromatography, ultrahigh pressure liquid chromatograph, quadrupole time-of-flight mass spectrometer (SFC/UHPLC Q-ToF/MS). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized and the ion masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with a supercritical chromatograph provides additional structural identification power by separating mixtures of compounds using high pressures and temperature before they reach the mass spectrometer. In the ToF method the mass-to-charge ratio of an ion is determined by the way of a time measurement in which ions are accelerated by an electric field of known strength. The acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse students in research and research training using this modern analytical technique. The instrument serves as a tool for broadening the participation of women and underrepresented minorities in science and engineering research at this Hispanic Serving Institution.The award of this unique mass spectrometer is aimed at enhancing research and education at all levels. It especially allows the use of electrophilic foot printing to identify protein conformational changes in complex media and the creation of branched dendrimeric architectures for energy transmission. The mass spectrometer aids the characterization of non-covalent interactions between proteins and structured or modified nucleic acids and the identification of aspartic acid epimerization in the aging proteome. The instrument is also used to study self-sorting, supramolecular catalysis and molecular dynamics as well as to investigate serine peptide assembly. The mass spectrometer also serves researchers studying structure and mechanism in tryptophan synthase and exploring olefin polymerization catalysts with ligands featuring weakly coordinating carborane anions. The instrumentation is also used in analyzing protein biogenesis and protein quality, using metabolomics for deciphering t-cell heterogeneity and using asymmetric methods and synthesis strategies for the study of neuroprotective limonoids. It is also employed to study effects of post-transcriptional and post-translational modifications on the dynamics of eukaryotic translation initiation and to obtain entioselectivity from gold(III) complexes.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.