RoL: RAISE: DESYN-C3: A platform for Modular Pseudo-Organelles for Compartmentalization and Control of Pseudo-Cell Processes

制造商培养细胞以生产疫苗和蛋白质药物。这些产物复杂且难以合成，因为它们由蛋白质组成。生长细胞对于生产来说不是很有效，因为细胞除了制造特定的产品外，还需要大量的能量和原材料来生长和维持自身。这种限制激发了人们对创造合成细胞以模拟支持产物形成而不是细胞生长的细胞过程的兴趣。一些细胞过程发生在单独的隔室中，以避免相互干扰。该项目将尝试创建可以发生生物反应的合成隔室。成功将使创造复杂的分子和蛋白质更容易。它还将增加我们对细胞如何执行和协调关键功能的理解。项目成果将通过视频与公众分享。实验室图尔斯参观将提供给女孩通过职业日的女孩计划在西北。来自芝加哥公立学校的少数民族学生也将得到指导。这些努力应该鼓励更多的学生在STEM相关领域从事职业。控制生物分子反应发生的时间和地点仍然是无细胞系统设计中的一个重大挑战。在这个项目中，将系统地探讨区室化在生物合成反应模型中的作用。将检查combustion以揭示空间分离和分离隔室的性质如何增强无细胞合成。这种方法将把区室化与无细胞代谢和调节技术结合起来。将采用计算建模来模块化地实现模型生物分子的产生。该项目成果的贡献将是通过整合区室化，受控运输，和细胞-该奖项反映了NSF的法定使命，并通过利用基金会的知识价值和更广泛的影响进行评估，被认为值得支持审查标准。

Manufacturers culture cells to produce vaccines and protein-based drugs. These products are complex and difficult to synthesize because they are composed of proteins. Growing cells is not very efficient for production because a cell uses a lot of energy and raw material to grow and maintain itself in addition to making a particular product. This limitation spurs an interest in creating synthetic cells to mimic the cellular processes that support product formation rather than cell growth. Some cellular processes occur in separate compartments to avoid interference with each other. This project will attempt to create synthetic compartments where biological reactions can take place. Success would make creating complex molecules and proteins easier. It would also increase our understanding of how cells perform and coordinate critical functions. The project results will be shared with the general public through videos. Lab tours will be provided to girls through the Career Day for Girls program at Northwestern. Underrepresented minority students from Chicago Public Schools will also be mentored. These efforts should encourage more students to pursue careers in STEM-related fields.Controlling when and where biomolecular reactions take place remains a significant challenge in the design of cell-free systems. In this project, the role of compartmentalization on a model biosynthetic reaction will be explored systematically. Compartments will be examined to uncover how spatial segregation and the nature of the segregating compartment can enhance cell-free synthesis. The approach will couple compartmentalization with techniques in cell-free metabolism and regulation. Computational modeling will be employed to modularly implement production of a model biomolecule. The contribution of the project results will be to markedly improve biological synthesis in cell-free environments by integrating compartmentalization, controlled transport, and cell-free expression systems to achieve greater temporal control and expand the environments in which such systems can be employed.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.