Compressed Sub-Aperture Super-Resolution Microscopy

研究人员建议利用光学显微镜领域的最新发展，并设计一种新型的反射模式成像仪器，该仪器集成了压缩传感能力，从而能够开发出一种可用于眼睛和其他生物目标超分辨成像的系统。扫描激光检眼镜（SLO）是视网膜诊断成像的支柱。它以共焦激光扫描显微镜为基础，最近与自适应光学技术相结合，提供更清晰的视网膜图像。该项目将1）开发使用光学重新分配反射共焦显微镜所需的概念和技术。这将导致用于超分辨视网膜反射成像的扫描激光检眼镜（ORSLO）。2)通过开发基于数字微镜技术的新型光学架构来增强ORSLO。这将允许视网膜感光细胞和脉管系统的可视化。3)将压缩传感技术应用于自适应光学扫描激光检眼镜（AOSLO）中，实现反射式图像扫描显微镜（ISM）。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

The investigators propose to take advantage of very recent developments in the field of optical microscopy and engineer a novel reflectance mode imaging instrument integrated with compressed sensing capability enabling the development of a system that can be used for super-resolved imaging of the eye and other biological targets. Scanning laser ophthalmoscopy (SLO) is a mainstay of diagnostic imaging of the retina. It is based on confocal laser scanning microscopy and has recently been combined with adaptive optics technology to provide sharper images of the retina.The project will 1) Develop the concepts and technology necessary to use optical reassignment reflectance confocal microscopy. This will lead to scanning laser ophthalmoscope (ORSLO) for super-resolved retinal reflectance imaging. 2) Enhance ORSLO by developing a novel optical architecture based on digital micro-mirror technology. This will permit the visualization of retinal photoreceptor cells and vasculature. 3) Implement reflectance image scanning microscopy (ISM) with a compressed sensing technique into an adaptive optics scanning laser ophthalmoscope (AOSLO). This will enable super-resolved adaptive optical imaging.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.