MRI: Acquisition of a Matrix-Assisted Laser Desorption/Ionization Time-of-Flight(MALDI TOF) Mass Spectrometer for of Research and Research Training at CSULA

该奖项由主要研究仪器计划支持。来自加州州立大学洛杉矶的Yong Ba教授和同事Jamil Momand、Edith Porter、Yixian Wang和Cecilia Momita-Lopez正在购买一台配备光电二极管阵列的基质辅助激光解吸/电离飞行时间质谱仪（MALDI-TOF-MS）。一般来说，质谱（MS）是用于识别和表征复杂样品中少量化学物质的关键分析方法之一。MALDI TOF结合了温和的离子化（非常适合产生肽、蛋白质、核酸、碳水化合物、合成聚合物和其他类似大小的物质的完整离子）和检测模式，在广泛的样品中提供灵敏度和准确度之间的出色平衡。此次收购加强了大学和区域内的研究基础设施。该工具通过向多样化的学生群体提供动手机会，扩大了参与。该奖项还用于外联活动，旨在加强各级的研究和教育。它特别影响与分子结构、取向和动力学相关的研究。 研究人员使用MALDI-TOF-MS来研究粘膜粘附共聚物胶体凝胶系统的相互作用，并在哺乳动物细胞中鉴定蛋白质精氨酸甲基转移酶7（PRMT 7）的体内底物和结合伴侣。质谱仪用于研究可能在帕金森病中起作用的细胞破坏机制，并用于研究抗冻蛋白。该仪器还为研究人员鉴定与t-Darpp AKT激活蛋白相关的蛋白质提供服务，其中t-Darpp是多巴胺调节的磷蛋白的截短同种型，具有结合钙的细长单体，并被细胞周期蛋白依赖性酶激酶磷酸化。最后，该仪器被研究抗菌肽的研究人员使用。 总体而言，仪器采购可能有利于国家健康和福利，以及支持教育和多样性。该奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

This award is supported by the Major Research Instrumentation Program. Professor Yong Ba from California State University Los Angeles and colleagues Jamil Momand, Edith Porter, Yixian Wang and Cecilia Zurita-Lopez are acquiring a matrix-assisted laser desorption / ionization time-of-flight mass spectrometer (MALDI-TOF-MS) equipped with a photodiode array. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide range of samples. The acquisition strengthens the research infrastructure at the university and within the regional. The instrument broadens participation by giving hands-on access to a diverse student population. The instrument is also used in outreach activities.The award is aimed at enhancing research and education at all levels. It especially impacts studies correlating molecular structure, orientation and dynamics. Researchers use the MALDI-TOF-MS to study interactions of a mucoadhesive co-polymer colloidal gel systems and in identifying in vivo substrates and binding partners for the protein arginine methyltransferase 7 (PRMT7) in mammalian cells. The mass spectrometer is used for studying cell disruption mechanisms that may be operational in Parkinson's disease and for studying antifreeze proteins. The instrument also serves researchers identifying proteins associated with t-Darpp AKT activation proteins, in which t-Darpp is a truncated isoform of dopamine-regulated phosphoprotein having an elongated monomer that binds calcium and is phosphorylated by cyclin-dependent enzyme kinases. Finally, the instrument is used by researchers investigating antimicrobial peptides. Overall, the instrument acquisition may benefit the National health and welfare as well as supporting education and diversity.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.