Patient-derived diseased and autologous immunocompetent skin models for the examination of drug-loaded nanocarrier systems (C02)

本项目一方面旨在通过使用毛囊来源的外根鞘细胞和来自同一供体的免疫细胞重建的自体人体免疫皮肤模型，研究纳米载体与皮肤免疫细胞之间的复杂相互作用。它将用于研究局部应用纳米载体与皮肤免疫细胞之间的相互作用。另一方面，将通过基因调节和/或来自牛皮癣患者的皮肤细胞来开发复杂的牛皮癣体外模型，从而确定体外药物效应，并检测载药纳米载体的潜在益处和副作用。

This project aims on the one hand to examine the complex interplay between nanocarriers and immune cells of the skin using an autologous, human-based immunocompetent skin model, which is reconstructed using hair-follicle derived outer root sheath cells and immune cells derived from the same donor. It will be used to study interactions between topically applied nanocarriers and skin immune cells. On the other hand a complex in vitro model of psoriasis will be developed via gene modulation and/or skin cells derived from psoriasis patients allowing one to determine drug effects in vitro and to detect the potential benefits but also side effects of drug-loaded nanocarriers.