Characterization of transport and release properties of responsive nanocarriers by time-resolved fluorescence spectroscopy/microscopy and localization microscopy (B03)

该项目旨在通过使用先进的时间分辨荧光方法，回答具有不同物理化学性质的反应性纳米载体药物偶联物在3D皮肤模型、人类和小鼠皮肤组织中作为物理和生化环境的功能渗透到皮肤中并在皮肤中起作用的问题。发展包括多参数和多光子荧光寿命成像（FLIM, FLIM- fret， PLIM）用于炎症皮肤的层析和生理分析。计划将单分子全内反射显微镜（TIRF）与FLIM相结合，用于毛囊内的单分子检测。

The project aims to answer the question by which responsive nanocarrier-drug conjugates with different physico-chemical properties penetrate into and act in the skin as a function of the physical and biochemical environment in 3D skin models, human, and murine skin tissue by using advanced time-resolved fluorescence methods. Developments include multiparameter and multiphoton fluorescence lifetime imaging (FLIM, FLIM-FRET, PLIM) for the tomographic and physiological analysis of inflamed skin. A combination of single molecule total internal reflection (TIRF) microscopy with FLIM for single molecule detection in the hair follicle planned.