DNA origami nanoarchitectures for predesigned spatial confinement of proteins (A06)

在这个项目中，我们的目标是开发一种通用的主客体络合策略，用于操纵特定封装到定制DNA笼中的所需蛋白质的稳定性和活性。为此，我们将使用具有明显特异性、亲和力和相关读出信号的超分子配体的大型工具。多价配体-蛋白质相互作用的空间控制也将被用来模拟动粒-微管界面基本成分的结构，从而能够更深入地了解染色体分离的机制，更广泛地为人工蛋白质机器的产生提供一种策略。

In this project we aim at developing a general host-guest complexation strategy for the manipulation of the stability and activity of desired proteins specifically encapsulated into customized DNA cages. To this end, we will use a large tool of supramolecular ligands with distinct specificity, affinity and associated read-out signals. Spatial control of multivalent ligand-protein interactions will be also employed to mimic the architecture of fundamental components of the kinetochore-microtubule interface, thus enabling to gain a deeper insight into the mechanisms of chromosome segregation and more in general to offer a strategy for the generation of artificial protein machineries.