Molecular specificity of signal input and signal output in plant receptor kinases (D05)

该项目旨在了解不同配体的特异性是如何由EFR、ELI(EFR-like)、CORE和XA21的结构高度相似的LRR结构域决定的。通过有针对性地改变这些LRR结构域的表面(通过LRR交换、合成LRRs的设计、定点突变)，功能相关的残基将被识别出来，如果可能，将用于创建新的合成配体-受体对。在第二部分，BRI1和EFR的模块化结构将被用于构建嵌合受体，我们的目的是阐明不同的细胞质信号输出程序(发育和防御)是如何确定的。

The project aims at understanding how specificity for distinct ligands is determined by structurally highly similar LRR domains of EFR, Eli (EFR-like), CORE and XA21. Using targeted changes of the surface in these LRR domains (by LRR swaps, design of synthetic LRRs, site-specific mutagenesis) the functionally relevant residues will be identified and, if possible, used to create novel, synthetic ligand-receptor pairs. In a second part, the modular architecture of BRI1 and EFR will be used for the construction of chimeric receptors with which we aim to elucidate how distinct cytoplasmic signal output programs (development versus defence) are determined.