Functional diversity in Drosophila glial cells, the role of schlaflos and rumpel

在神经系统中，几类神经胶质细胞为正常的大脑功能提供了一系列功能。在目前的建议中，我们的目标是破译不同果蝇神经相关胶质细胞的功能异质性。我们确定了编码膜相关蛋白的基因，这些蛋白是果蝇正常运动所需的不同神经相关胶质细胞所必需的。schlaflos （CG6927）编码一种预测的GABAA/甘氨酸受体，是果蝇活动行为所必需的。该基因仅在少数与神经丸相关的神经胶质细胞中表达，这将进一步表征。rumpel （CG9657）编码一种与哺乳动物SLC5A家族同源的钠偶联单羧化转运蛋白，并在不同的神经胶质细胞亚群中特异性表达。神经胶质瘤功能是抑制有害温度刺激下的神经元活动所必需的。我们将产生基因突变，并在SPP1757的框架内解剖其在神经胶质生理学中的功能。

Within the nervous system several classes of neuropil glia provide a range of functions for normal brain functionality. In the current proposal we aim to decipher the functional heterogeneity of the different Drosophila neuropil associated glial cells. We identified genes encoding membrane associated proteins that are required in distinct neuropil associated glial cells for normal locomotion of Drosophila flies. schlaflos (CG6927) encodes a predicted GABAA/glycine receptor and is needed for the activity behavior of the flies. The gene is expressed in only few of the neuropil-associated glial cells which will be further characterized. rumpel (CG9657) encodes a sodium-coupled monocarboxylat transporter homologous to the mammalian SLC5A family and is specifically expressed in a different subset of neuropil glial cells. The glial rumpel function is required to dampen neuronal activity upon noxious temperature stimuli. We will generate mutants of genes and will dissect their functions in glial physiology in the framework of the SPP1757.