Posttranscriptional regulatory interactions during Herpes Simplex Virus infection

疱疹病毒引起多种人类疾病，治疗或预防的选择往往非常有限。因此，需要提高我们对这些病毒复制策略的了解，以确定新的干预目标。在这里，我们想分析单纯疱疹病毒（HSV）感染细胞中mRNA和蛋白质之间的动态相互作用。我们建议系统地剖析HSV感染过程中的转录后调控，包括其分子组成和结构以及其生物学功能。我们打算发现宿主和病毒mRNA相互作用的蛋白质，在病毒复制过程中表现出差异结合，以确定其目标转录本，并表征这些蛋白质-RNA相互作用如何影响细胞和病毒mRNA的命运。我们的研究结果将有助于将转录后相互作用整合到其他细胞基因调控网络中，并通过识别影响病毒复制的新型RNA结合蛋白来实现转化医学的潜力。

Herpes viruses cause a variety of human diseases with often very limited options for treatment or prevention. Thus, there is a need to improve our understanding of the replication strategies of these viruses to identify novel targets for intervention. Here, we want to analyze the dynamic interplay between mRNAs and proteins in Herpes Simplex Virus (HSV) -infected cells. We propose to systematically dissect posttranscriptional regulation during HSV infection concerning its molecular composition and architecture as well as its biological functions. We intend to discover host and viral mRNA-interacting proteins that show differential binding during viral replication, to identify their target transcripts and to characterize how these protein-RNA interactions influence the fate of cellular and viral mRNAs. Our results will be instrumental to integrate posttranscriptional interactions into other cellular gene regulatory networks as well as carry the potential for translational medicine by identifying novel RNA-binding proteins, which influence viral replication.