Sequence-controlled, multifunctional materials by living anionic polymerization of aziridines

提出了一种序控阴离子聚合叠氮嘧啶的方法。为了实现阴离子开环聚合，氮化吡啶必须被磺胺类药物激活。首次对不同的磺胺类化合物的聚合行为进行了研究，并首次提出了用于阴离子聚合的功能氮化吡啶单体。磺胺有两个功能：首先是去除酸性质子，即作为保护基团；其次是通过电子抽离激活环。活化对活性链端（偶氮阴离子）的反应性有直接影响，这将允许根据活化基团的类型进行第一次序列控制的阴离子聚合。将不同的官能团连接到叠氮上（在环上），并研究聚合行为。所得到的聚磺胺将被水解释放出相应的多胺。将对这些材料的DNA转染活性进行研究。建立聚合物（序列）结构与DNA转染活性的关系，并与常规的非病毒基因转染进行比较。

The proposal presents the sequence-controlled anionic polymerization of aziridines. To enable anionic ring-opening polymerization, the aziridine has to be activated by sulfonamides. For the first time different sulfonamides will be investigated with respect to their polymerization behavior and the first functional aziridine monomers for anionic polymerization are presented. The sulfonamide serves two functions: first to remove the acidic proton, i.e. as a protection group, and second to activate the ring by electron withdrawal. The activation has a direct influence on the reactivity of the active chain end (an azaanion), which will allow the first sequence-controlled anionic polymerization depending on the type of the activation group. Different functional groups will be attached to the aziridine (at the ring) and the polymerization behavior will be investigated. The resulting polysulfonamides will be hydrolyzed to release the respective polyamines. These materials will be investigated with respect to their DNA transfection activity. The establishment of a polymer (sequence) structure- DNA transfection activity relation will be conducted and compared to conventional non-viral gene transfection.