Multistep chip reactors with an integrated continuous separation

在项目7中，正在开发基于芯片的多步流动反应器，该反应器允许在两个阶段中连续合成，中间为微型自由流动电泳。将追求的组合微反应器microFFE平台的有机合成的制造，适应和应用。为了方法开发，喹啉取代的环戊烯的合成进行了研究。合成的主要产物将在microFFE中与副产物分离。随后，将进行第二反应器阶段下游到制备分离的整合，其适合于升高的温度并且允许在微尺度上控制和监测温度。在该流动反应器阶段中，进行Diels-Alder反应以在喹啉侧链中建立多环体系。将开发用于在线监测化学反应和分离的UV区域中的光学荧光检测，并且将多级反应器耦合到（生物）阻抗谱将被证明能够实现集成的生物传感器。通过这些功能的集成，将首次实现在单个微流控芯片上进行连续多步有机合成，这需要一个分离步骤，并实现在线监测和生物测试。

In Project 7 chip-based multistep flow reactors are being developed, which allow a continuous synthesis in two stages with an intermediate micro free flow electrophoresis. The fabrication, adaptation and application of combined microreactor-microFFE platforms for organic synthesis will be pursued. For method development the synthesis of quinoline-substituted cyclopentenes is investigated. The main product of the synthesis is to be separated from a by-product in the microFFE. Subsequently, the integration of a second reactor stage downstream to the preparative separation, which is suitable for increased temperatures and which allows to control and monitor the temperature on the micro scale, will be performed. In this flow reactor stage a Diels-Alder reaction to build up a multicyclic system in the quinoline side chain is to be carried out. Optical fluorescence detection in the UV region for inline monitoring of chemical reactions and separations will be developed and the coupling of multistage reactors to (bio-)impedance spectroscopy will demonstrated to enable an integrated biosensor. Via the integration of these functions, a continuous multistep organic synthesis, which requires a separation step, on a single microfluidic chip and its inline monitoring and biological testing will be enabled for the first time.