Engineering of biofunctionalized vascular grafts using elektrospinning of human extracellular matrix and additional oligonucleotid coating for capturing of circulating endothelial progenitor cells (EPC)

目前治疗动脉瘤或狭窄的大直径血管移植物使用两种不同的假体材料。涤纶人工血管具有良好的血液相容性，但存在持续感染风险、不能生长或重塑以及假性内膜剥落等缺点。替代使用的同种异体血管移植物减少了剥离和感染的风险，因此被广泛用于替换感染的人造血管移植物。此外，由于器官稀缺，这些同种异体移植物的可用性有限，这些移植物往往会严重钙化，特别是在儿科人群中。最佳的动脉移植材料由具有天然动脉特征的人体组织成分组成，包括内皮细胞衬里。聚四氟乙烯人工血管的自体内皮衬里用于外周血管和冠状动脉搭桥术时，显示了成功的短期和中期结果。然而，相当复杂的体外制造伴随着感染风险。该资助计划的目的是设计一种现成的血管移植，使用人杂合细胞外基质成分(ECM)的电纺丝，并使用寡核苷酸进行额外的表面生物功能化，以吸引和结合循环内皮祖细胞，以便在植入后进行体内自体内皮化。

The current treatment of aneurysatic or stenpotic large diameter vascular grafts uses two different prosthetic materials. Dacron prothesis serve the purpose of a blood vessels with good hemocompatibility however have the disadvantages of a constant infection risk, an inability to grow or remodell and pseudointimal peeling. Alternatively used allogenic vascular grafts reduce the risk for peeling and infection and are accordingly broadly used for replacement of infected prosthetic vascular grafts. Beside a limited availability due to organ scarcity these allografts tend to calcify heavily in particular in the pediatric population. Optimal arterial graft materials consist of human tissue components with characteristics of native arteries including an endothelial cell lining. Autologous endothelial lining of PTFE-prosthesis showed successfull short- and mid-term results when used for peripheral vascular and coronary artery bypass grafting. The rather complex in vitro manufacturing is however accompanied with an infection risk. Objective of the grant proposal is the engineering of an off-the-shelf vascuar graft using elektrospinning of human hybrid extracellular matruix components (ECM) with an additional surface biofunctionalization using oliginucleotids in order to attract and bind circulating endothelial progenitor cells for an autologous in vivo endothelialization after implantation.