Initial steps of thylakoid membrane formation and the role of endomembrane system-mediated protein targeting into plastids (B10)

项目B10旨在确定在高等植物叶绿体中形成高度组织的类囊体网所需的成分和基本步骤。一个中心方面是通过利用先前确定的类囊体类囊体生物发生因子HCF222来研究内膜系统对类囊体体形成的贡献。我们的数据表明，该蛋白通过膜系统介导的途径定位于叶绿体。利用遗传学方法，我们打算确定这一非规范的输入途径中涉及的特定成分和要求，以及它对类囊体体发育的影响。此外，我们将使用反向遗传方法来分离参与类囊体膜形成早期步骤的新蛋白，重点放在内膜的过程中。

Project B10 aims to identify components and fundamental steps required for the formation of the highly organized thylakoid network in higher plant chloroplasts. One central aspect is to investigate the contribution of the endomembrane system to thylakoid formation by exploiting the previously identified thylakoid biogenesis factor HCF222. Our data show that this protein is targeted to chloroplasts via the endomembrane system-mediated pathway. Using a genetic approach, we intend to identify specific components and requirements involved in this non-canonical import pathway and its impact on thylakoid development. Moreover, we will use reverse genetic approaches to isolate novel proteins involved in early steps of thylakoid membrane formation, focusing on processes at the inner envelope.