Synthetic genetic analysis, automated microscopy and image analysis (Z03)

对于所有使用酵母作为模式生物的项目(P04-Schwappach、P06-Thumm和P13-Rehling)，该技术项目将使用合成遗传阵列(SGA)技术提供定制的突变酵母菌株文库。这些图书馆将允许单个项目推动其基于假设的研究计划。系统的分析将有助于解决CRC的生物学问题，因为我们对间隔门功能的洞察受到极少数模型货物蛋白的广泛使用的限制。在隔间联络点领域，这种做法最近促进了重大进展，并将继续这样做。中央平台提供阵列钉扎机器人、液体处理机器人、自动显微镜和图像分析流水线的组合，非常适合在酵母中进行系统的定位筛选。此外，该平台将支持P07(Kehlenbach)和P10(BRose)在哺乳动物细胞中实施定量成像分析，与手动显微镜和图像分析相比，吞吐量大幅提高。这个中央项目使用现有的尖端设备，为儿童权利委员会提供强大的试剂和方法。

For all projects using yeast as a model organism (P04 - Schwappach, P06 - Thumm and P13 - Rehling) this technical project will provide tailor-made libraries of mutant yeast strains using synthetic genetic array (SGA) technology. These libraries will allow the individual projects to drive their hypothesis-based research program. Systematic analysis will be an asset to the biological problems of the CRC because our insight into compartmental gate function is limited by the extensive use of very few model cargo proteins. In the area of compartmental contact sites such approaches have recently spurred major advances and will continue to do so. The central platform provides a combination of array pinning robot, liquid handling robot, automated microscope, and image analysis pipeline ideally suited to perform systematic localization screens in yeast. Furthermore, the platform will support P07 (Kehlenbach) and P10 (Brose) to implement quantitative imaging assays in mammalian cells with substantially increased throughput as compared to manual microscopy and image analysis. This central project uses existing cutting-edge equipment to provide powerful reagents and methodology to the CRC.