Stringent and heat stress response in Bacillus subtilis

枯草芽孢杆菌的严格反应和热胁迫反应我们发现，在枯草芽孢杆菌的耐热性过程中，严格反应是热应激反应的一部分。我们推测，导致第二信使下调翻译、细胞生长和DNA复制的已知严格反应活动是热应激反应中复杂而重要的部分，因为抑制新的蛋白质合成将降低细胞蛋白质质量控制系统的负荷。这表明细菌细胞可能遵循一种策略，即上调细胞蛋白质质量控制系统，同时下调新的蛋白质合成，这已经在真核细胞的未折叠蛋白质反应中观察到。这是一个非常有趣的新方面，在细菌中并不是真正广为人知的特征。采用这样一种快速有效的调控机制在生物学上是有意义的，在这种机制中，第二信使的水平可以直接控制，它可以立即影响许多不同的细胞过程。在我们的优先项目1879核苷酸第二信使信号在细菌中的项目提案中，我们希望与同事合作，阐明严格反应在感知和响应热应激中的分子机制和作用，以及与同时激活的Spx介导的应激反应途径有关的分子机制。

Stringent and heat stress response in Bacillus subtilis We identified stringent response as part of heat stress response during thermotolerance in B. subtilis. We hypothesize that the known activities of stringent response, which result in the second messenger down regulation of translation, cell growth and DNA replication, is an intricate and important part of heat stress response, because curbing new protein synthesis will lower the load on the cellular protein quality control system. This suggests that bacterial cells might follow a strategy of upregulation of the cellular protein quality control system combined with a downregulation of new protein synthesis, already observed for the eukaryotic unfolded protein response. This is a very interesting new aspect not really well known and characterized in bacteria. It would make biological sense to adopt such a very fast and efficient regulatory mechanism, where the level of a second messenger, which can influence instantly many different cellular processes, is directly controlled. In our project proposal for the priority program 1879 Nucleotide Second Messenger Signaling in Bacteria we want to elucidate in collaboration with colleagues the molecular mechanism and the role of the stringent response in sensing and responding to heat stress also in relation to the concurrently activated Spx mediated stress response pathway.