Needle-free microfluidic vaccine/drug delivery

2022年（R4）的研究目标是研究纳秒脉冲电场诱导的空化/冲击波流动微射流，定量评价离体和体外输送，并发表论文。根据该计划，设计并测试了具有最小腐蚀的电极和适合纳秒脉冲放电微射流的喷嘴结构。去年建造的超高速实时高度放大光学装置用于评估不同施加能量的放电诱导微流的微流体和流动物理。已经编写了一份成果手稿，准备提交给一份影响力很大的期刊。进行实验以评估在离体疫苗/药物递送中的微射流穿透深度和体积。为了定量评价微射流在皮肤、软组织和肿瘤模型中的体外和离体显微成像，我们对微射流在皮肤、软组织和肿瘤模型中的作用进行了研究。进行了体内实验，以了解微射流和冲击波微流对细胞的影响。有趣的结果发表在一篇文章中，补充手稿已经编写并正在提交中。离体样本的组织学评价正在进行中。

The goal of the research for year 2022 (R 4) was defined as investigation of nanosecond-pulsed-electric-field induced cavitation/shock wave streaming microjet; quantitative evaluation of in-vitro and ex-vivo deliveries; and publication.According to the plan, electrodes with minimize erosion and appropriate nozzle configuration for nanosecond pulse discharge microjet delivery were designed and tested. The ultra-high-speed real-time highly magnified optical setup, which was constructed last year, was used to evaluate the microfluidic and flow physics of the electric discharge induced microstreaming for different applied energies. A manuscript from results has been prepared for submission to a high impact journal. Experiments were performed to evaluate microjet penetration depth and volume in ex-vivo vaccine/drug deliveries. Experiments have been continued to conjugate new protein nanoparticles to minimize the drug/vaccine volume, a new manuscript from the results has been prepared.For quantitative evaluation, in-vitro and ex-vivo microscopic visualization of microjets in skin, soft tissue, and tumor models were performed. In-vivo experiments were performed to understand effects of microjet and shock waves microstreaming on cells. The interesting results was published in an article, additional manuscript has been prepared and is under submission. Histological evaluations of ex-vivo samples have been underway.