Bimodal Imaging of CAR-expressing natural killer cells via PET/MRT

本研究的目的是建立和验证89-Zr标记的SPIO，用于肝癌大鼠模型中表达CAR的NK细胞系的双峰成像。该项目的主要方面是对瘤内注射后肿瘤环境中表达CAR的NK细胞系(NK-92/31.28z.IEW)进行定量。对阵静脉注射。通过PET-示踪剂标记的超顺磁性粒子(SPIO)进行应用。对于临床前成像，放射性同位素-89被结合在粒子的表面。在细胞系积累的初步结果出来后，将通过DWI-MRT和FDG-F18PET检测免疫治疗效果。

The aim of the study is the development and validation of 89-Zr-labeled-SPIO for bimodal imaging of a CAR-expressing NK cell line in a HCC rat model. The main aspect of the project is the quantification of the CAR-expressing NK cell line (NK-92/31.28z.IEW) in the tumor environment after i.a. versus i.v. application via PET-Tracer labeled superparamagnetic particle (SPIO). For the preclinical imaging the radioisotope Zirconium-89 is bound on the surface of the particle. After the initial results of the accumulation of the cell line, the immuntherapeutical effect will be detected via DWI-MRT und FDG-F18 PET.