Analysis of co-translationally acting factors regulating the biogenesis of chloroplast encoded proteins (A05)

我们发现，热驯化导致特定的叶绿体编码的光系统II核心蛋白的合成重塑。此外，我们揭示了一个广泛的网络的核糖体相关因子的叶绿体，含有许多假定的调制器和整合剂与改变核糖体结合的行为在热驯化。基于这些发现，该项目旨在加深对温度驯化过程中蛋白质生物合成如何调节的理解。我们计划研究选定的推定调节剂对调节翻译本身的贡献，并研究共翻译新生多肽加工和膜整合的核心步骤。

We found that heat acclimation leads to remodelling of the synthesis of specific chloroplast-encoded photosystem II core proteins. Furthermore, we revealed a broad network of ribosome-associated factors in chloroplasts, containing many putative modulators and integrators with altered ribosome-binding behaviour during heat acclimation. Based on these findings, the proposed project aims to deepen the understanding how protein biogenesis is regulated during temperature acclimation. We plan to investigate selected putative modulators for their contribution for tuning translation itself and to examine central steps of co-translational nascent polypeptide processing and membrane integration.