Effect of milk and milk components on the in-situ pellicle

龋齿和侵蚀的发生是由于牙齿表面的生物粘附过程。在这种情况下，外膜被认为是发生在牙硬组织上的生理和病理生理过程的关键介质。由于特定唾液成分的选择性吸附，口腔内所有坚硬表面的膜在很短的时间内形成。通过应用生物或仿生制剂有意修饰这一生理蛋白和糖蛋白层，以改善保护膜的特性，似乎是一种创新的前瞻性口腔预防方法。在这种背景下，有必要注意到牛奶含有各种酪蛋白，由于它们的胶束组织似乎与参与膜形成的某些成分相似。因此，天然酪蛋白和改性酪蛋白的吸附似乎是一种可能的尝试，以提高原位膜的龋和侵蚀保护潜力。此外，酪蛋白胶束可以作为潜在的纳米载体应用于牙齿表面的保护成分。然而，酪蛋白与原位膜的相互作用尚未得到详细的研究。因此，本研究旨在探讨不同类型的牛奶、牛奶成分和牛奶衍生物对原位膜的影响。重点将放在超微结构修饰的透射电镜研究以及酪蛋白在原位膜上的吸附；ELISA法测定酪蛋白积累量。此外，将通过基于荧光的方法确定选定漂洗剂的初始细菌定植。这些研究将从根本上有助于理解口腔生物粘附过程的改变。此外，将为预防性牙科创新生物学方法的发展奠定基础。

The incidences of caries and erosion are attributed to bioadhesion processes at the tooth surface. In this context, the pellicle is regarded as the crucial mediator for the physiological and pathophysiological processes occuring on the dental hard tissues. Due to the selective adsorption of specific salivary components the pellicle is formed within a very short time on all hard surfaces in the oral cavity. The intentional modification of this physiological protein- and glycoprotein layer by the application of biological or biomimetic preparations in order to improve the protective pellicle properties appears to be an innovative prospective approach in oral prophylaxis. Against this background, it is necessary to note that milk contains various caseins which due to their micellar organization appear to resemble certain components involved in pellicle formation. Hence, the adsorption of native as well as modified caseins seems to be a possible attempt to improve the caries- and erosion protective potential of the in-situ pellicle. Furthermore, casein-micelles could serve as potential nanocarriers for the application of protective components at the tooth surface. However, the interaction of caseins with the in-situ pellicle has so far not been investigated in detail. Therefore, the proposed clinical-experimental study aims to examine the effect of different types of milk, milk components and milk derivatives on the In-situ pellicle. Emphasis will be given to transmission electron microscopic investigation of ultrastructural modifications as well as the adsorption of caseins to the in-situ pellicle; the casein-accumulation will be quantified by ELISA. Additionally, initial bacterial colonization will be determined for selected rinses by fluorescence-based methods. The investigations will contribute fundamentally to the understanding of the modification of oral bioadhesion processes. Furthermore, a basis for the development of innovative biological approaches in preventive dentistry will be created.