Characterization of the interaction of polysaccharides with dental materials including consecutive or simultaneous adsorption of proteins

多糖与牙科材料相互作用的表征，包括连续或同时吸附蛋白质

• 批准号: 392066266
• 负责人: Professor Dr. Matthias Hannig
• 金额: --
• 依托单位: Klinik für Zahnerhaltung, Parodontologie und Präventive Zahnheilkunde
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/392066266?language=en
• 关键词: Characterization; interaction; polysaccharides; dental; materials

Polysaccharides are one of the main classes of biopolymers, together with proteins, lipids and nucleic acids. Polysaccharides are on the one hand main constitutents of all living organisms and play, on the other hand, an important role in bacterial adhesion and biofilm formation. However, studies about carbohydrate interaction with solid substrates is very much underrepresented in literature if compared to the other natural biopolymers, i.e. nucleic acids and proteins. Although there were efforts to understand the biological function of carbohydrates, there is a lack of investigations of their interaction with solid substrates. In particular there are no systematic experiments with respect to type of carbohydrate and chain length/molecular weight. The aim of this project is thus to undertake for the first time systematic studies on the importance of carbohydrates and in particular the interaction between carbohydrates and proteins in the context of the initial bioadhesion processes on solid substrates. For this purpose, we will elucidate in detail the initial interaction of polysaccharides such as dextrans of different molecular weight and alginate, with surfaces of dental materials. The involved processes and forces will be studied as a function of the clinically relevant parameters pH, material, concentration of Ca ions and in particular proteins also present in the solution to find out which influence they have on the adsorption of polysaccharides, because in preliminary experiments we could find changes in composition and total mass if proteins were added to the experiment.

多糖是生物聚合物的主要类别之一，与蛋白质、脂类和核酸并列。多糖一方面是所有生物体的主要成分，另一方面在细菌黏附和生物被膜的形成中发挥着重要作用。然而，与其他天然生物聚合物，即核酸和蛋白质相比，文献中关于碳水化合物与固体底物相互作用的研究非常少。尽管人们努力了解碳水化合物的生物学功能，但缺乏对其与固体底物相互作用的研究。特别是，没有关于碳水化合物类型和链长/分子量的系统实验。因此，该项目的目的是首次系统地研究碳水化合物的重要性，特别是碳水化合物与蛋白质在固体基质上最初的生物黏附过程中的相互作用。为此，我们将详细阐述不同分子量的葡聚糖和藻酸盐等多糖与牙科材料表面的初步相互作用。所涉及的过程和力将作为临床相关参数的函数进行研究，pH，材料，钙离子的浓度，特别是蛋白质也存在于溶液中，以找出它们对多糖吸附的影响，因为在初步实验中，如果将蛋白质添加到实验中，我们可以发现组成和总质量的变化。

项目成果

Determination of the Amount of Carbohydrates Adsorbed on Solid Substrates

• DOI: 10.1002/pssa.201700845
• 发表时间: 2018-08
• 期刊: physica status solidi (a)
• 作者: Pariyanka Arenja;C. Rösch;Katharina Huttenlochner;C. Müller‐Renno;M. Hannig;C. Ziegler

Simultaneous quantification of total carbohydrate and protein amounts from aqueous solutions by the sulfuric acid ultraviolet absorption method (SA-UV method).

• DOI: 10.1016/j.aca.2021.338712
• 发表时间: 2021-08
• 期刊: Analytica chimica acta
• 影响因子: 6.2
• 作者: Swen Ehnert;Jürgen Seehase;C. Müller‐Renno;M. Hannig;C. Ziegler