Budding and asymmetric cell division in Hyphomonas neptunium (P04)

在细菌中，PARB同源物在将复制的DNA分配给子细胞的过程中发挥着核心作用。然而，它们的功能背后的确切机制仍不清楚。我们和其他人最近的工作表明，该蛋白家族的成员结合并水解核糖核苷酸CTP，使其可逆地与DNA结合，从而构成一类新的核苷酸依赖的分子开关。在这个项目中，我们在这些进展的基础上，旨在全面和定量地了解PARB在染色体分离中的作用以及这一过程与细胞分裂的协调。此外，我们将着手鉴定到目前为止尚未鉴定的蛋白质，这些蛋白质具有PARB的开关样特性，但控制不同于DNA分离的细胞过程。总之，这些研究将为CTP依赖的分子开关在细菌细胞生物学中的作用提供详细的见解。

In bacteria, ParB homologs play a central role in the partitioning of replicated DNA to the daughter cells. However, the precise mechanism underlying their function still remains unclear. Recent work from our group and others revealed that members of this protein family bind and hydrolyze the ribonucleotide CTP to bind reversibly to DNA, thus constituting a new class of nucleotide-depen¬dent molecular switches. In this project, we build on these advances and aim to obtain a compre¬hensive and quantitative mechanistic understanding of the role of ParB in chro-mosome seg¬regation and the coordination of this process with cell division. Moreover, we will set out to identify thus-far uncharac¬ter¬ized proteins that share the switch-like properties of ParB but control cellular pro¬cesses different from DNA segregation. Together, these studies will provide detailed insight into the role of CTP-dependent molecular switches in bacterial cell biology.