Studies on molecular and ecological genetics of in situ conservation of genetic resources of 7 species in genus Quercus

1)在serrata和Q.crispula 8个酶系统（GOT、LAP、AAP、GDH、ACO、GK、POD和GR）中检测到9个位点。在18个酶系统中检测到18个位点（除了前面描述的PGI、DIA、6PGE、MDH、ShDH、PGM、AMY、G6PD、MNR和ADH），对Q.phillyreoides、Q.acuta、Q.glauca、Q.myrisinaefolia进行了检测。被检测位点的酶系统存在较大差异。2)对serrata(4)和crispula(3) 8个居群及两种混交林的遗传参数进行分析，居群内遗传变异占总变异的98%。对各种群等位基因频率的遗传距离进行聚类分析，两种物种均被完全区分。3)利用9个、8个、7个和11个基因座对19个居群进行了群体遗传分析。同一种序的平均杂合度分别为0.408、0.321、0.171和0.146，表明前两种差异较大。居群间变异占总变异的10.5%。而在其他3种中，居群间变异率仅为3%。4)在毛茛、青花、豆蔻和尖叶毛茛中，亚属间变异占总变异率的23.4%，且毛茛与其他3种有较远的亲缘关系。5)青花青、豆蔻青和尖刺青的种间变异占总变异的36.9%，种内居群间变异仅占1%。6)由于居群间变异较大，因此在遗传资源保护方面，选择居群具有重要意义。

1) For Quercus serrata and Q.crispula, 9 loci in 8 enzyme systems (GOT,LAP,AAP,GDH,ACO,GK,POD AND GR) were detected. For Q.phillyreoides, Q.acuta, Q.glauca, Q.myrisinaefolia, 18 loci in 18 enzyme system (in addition to former described ones, PGI,DIA,6PGE,MDH,ShDH,PGM,AMY,G6PD,MNR,AND ADH) were detected. There were large diffrence in the enzyme systems being detected loci.2) In 8 populations of Q.serrata (4) and Q.crispula (3) and a mixed stand of both species were analyzed on populational genetic parameters, 98% of the total variation was retained within population. In cluster analysis of genetic distances with allele frequency of each population, both species were completely discriminated.3) In 19 populations of Q.phillyreoides (5), Q.glauca (6), Q.myrisinaefolia (2) and Q.acuta (6), populational genetic analyzes were made using 9,8,7 and 11 loci respectively. Mean heterozygosities were 0.408,0.321,0.171 and 0.146 in the same species order showing larger variation in the former two species. In Q.phillyreoides, 10.5% of total variation was detected among populations. Meanwhile, in other 3 species, only 3% of the variation was seen among populations4) In Q.phillyreoides, Q.glauca, Q.myrisinaefolia and Q.acuta, 23.4% of total variation was detected among sub-genus, and Q.phyllyreoides was found to be related remotely to other 3 species.5) In Q.glauca, Q.myrisinaefolia and Q.acuta, 36.9% of the total variation was found to be among species, and only 1% variation was retained among populations within species.6) For the genetic resource conservation of Q.phillyreoides and Q.glauca, it was found to be important to select population, because they have fairly large variation among populations.