Optimising the allocation of phosphorus fractions in rice to improve

本项目的主体实验--大型水培实验已顺利完成。自上次报告以来，该实验的所有实验室分析也已完成。这包括分析化学P组分浓度，在西澳大利亚大学显微镜、表征和分析中心（CMCA）分析细胞水平的P浓度，以及制备和交付样品给奈良科学技术研究所（NAIST）的合作者进行代谢物分析。生理、生物量、总磷和磷组分数据已合并成一份手稿，目前正在编写中，将提交给新植物学家。细胞水平的营养数据已进行分析，将形成第二份手稿，目前正在编写中。尚未收到代谢物数据，但我们预计将形成第三份手稿。此外，我与另外两名JSPS研究员（Asad Adhan和Getnet Adem）合作进行了一项联合实验，评估不同形式的P-应激下代谢物，蛋白质和基因表达的短期变化。这项实验已经成功完成，我们目前正在处理样品。总之，主要实验的样品已经全部分析或提交分析。两份手稿正在编写中，第三份正在等待结果。

The main experiment of this project, a large-scale hydroponic experiment has been successfully completed. Since the last report, all of the laboratory analysis from this experiment has also been completed. This included analysis of chemical P-fraction concentrations, analysis ofcell-level P concentrations at the Centre for Microscopy, Characterisation and Analysis (CMCA), University of Western Australia, and the preparation and delivery of samples to collaborators at Nara Institute of Science and Technology (NAIST) for analysis of metabolites. The physiological, biomass, total P, and P-fraction data have been combined into one manuscript, currently in preparation, to be submitted to New Phytologist.The cell-level nutrient data has been analysed and will form a second manuscript, currently in preparation. The metabolite data is yet to be received, but we expect it to form a third manuscript. In addition, I collaborated with two other JSPS fellows (Asad Prodhan and Getnet Adem) on a joint experiment assessing short-term changes in metabolites, proteins and gene expression, under different forms of P-stress. This experiment was completed successfully, and we are currently processing samples.In summary, samples from the main experiment have all been analysed or submitted for analysis. Two manuscripts are in preparation, and the third is awaiting results.