Analysis of bone quality in the elderly: effects of immobilization and type 2 diabetes mellitus as age-related risk factors

骨形态和组成的病理变化强烈影响骨的抗骨折能力。随着年龄的增长，这些变化变得越来越重要，因为由于老年人口的扩大和预期寿命的延长，骨折正在成为一个主要的公共卫生问题。揭示骨骼老化如何伴随常见的伴随条件，如固定和2型糖尿病改变骨组织的质量和影响其抗骨折是我们当代的重要目标。拟议研究计划的目的是确定和量化相关的、可测量的影响，这些影响会增加老年人的骨折风险。骨质量被定义为有助于骨骼固有抗骨折性的所有特征的总和。在这种情况下，骨细胞的状态及其微观结构、力学性能和生化组成对骨质量的维持起着重要作用。我们将对股骨皮质骨的组成和结构进行高分辨率分析-股骨是一个常见的骨折部位。样本将在死后从受固定和2型糖尿病影响的组织供体中获得。通过对个体骨层次水平的针对性分析，我们旨在探讨固定和2型糖尿病对骨稳定性、强度和抗骨折性的影响。我们的目标是研究细胞网络的功能和完整性，并特别关注其与矿物质和胶原蛋白特征的关系，从而增加对老年人复杂多维变化的理解。在拟议的项目中，临床分析工具和高分辨率表征技术的跨学科结合可以用有价值的新发现补充临床结果。因此，我们的工作计划不仅有助于骨科领域的发展，而且为寻找新的诊断工具和治疗方案提供了创新的视角，为老年人因固定和2型糖尿病而导致的骨质量改变提供了新的诊断工具和治疗方案。

Pathological changes in bone morphology and composition strongly influence bones fracture resistance. These changes gain importance with age since bone fractures are emerging as a major public health problem due to the expanding elderly population and increased life expectancies. Unraveling how skeletal aging accompanied by common concomitant conditions such as immobilization and type 2 diabetes mellitus alter the quality of bone tissue and influence its resistance to fracture is our important contemporary goal. The objective of the proposed research program is to identify and quantify relevant, measurable influences that amplify fracture risk in aged individuals.Bone quality is defined as the sum of all characteristics that contribute to the inherent fracture resistance of bone. In this context, the status of the bone cells, along with microstructure, mechanical properties and biochemical composition, play an important role since they actively contribute to the maintenance of bone quality. We will perform high-resolution analyses of the composition and structure of cortical bone from femora - a frequent fracture site. Samples will be obtained post mortem from tissue donors affected by immobilization and type 2 diabetes mellitus. With our targeted analysis of the individual hierarchical levels of bone, we aim to investigate the influences that endanger the stability, the strength and the resistance to fracture under immobilization and type 2 diabetes mellitus. Our goal is to examine the function and integrity of the cellular network and specifically focus on its relation to the mineral and collagen characteristics, thereby increasing the understanding of the complex multidimensional changes in aged individuals. The cross-disciplinary combination of clinical analytic tools and high-resolution characterization techniques in the proposed project can complement results from the clinics with valuable new findings. Thus, our work program will not only contribute to the advancement of the field of osteology but provide innovative perspectives for the search for new diagnostic tools and treatment options for elderly individuals with bone quality changes due to immobilization and type 2 diabetes mellitus.