Isolation of monochlorophenol-dechlorinating microorganisms using bio-electrochemical enrichment system with plug flow under anaerobic conditions

厌氧脱氯是氯代芳香族化合物生物修复中的重要反应，可降低其持久性和毒性。厌氧脱氯反应器的分离研究已有很多报道。然而，除了少数例外，一氯酚脱氯厌氧微生物尚未被分离。本研究对一氯酚脱氯厌氧微生物进行了分离。生物电化学富集系统或厌氧推流式富集柱系统对单氯酚的脱氯效果不明显，但对多氯酚的脱氯效果明显。不同条件下的试验表明，一氯酚脱氯培养不需要乳酸、甲酸等其他碳源。因此，我们提供了以一氯酚或苯酚为唯一碳源和能源的厌氧富集系统。富集苯酚导致在硫酸盐还原条件下生长在苯酚降解上的厌氧菌的分离。成功地获得了3-氯酚的富集物，并鉴定了3-氯酚脱氯厌氧菌。这些发现扩展了我们对单氯酚脱氯剂及其特性的认识。

Anaerobic dechlorination is the important reaction in the bioremediation of chlorinated aromatic compounds for decreasing persistency and toxicity of the compounds. There are many reports on the isolation of anaerobic dechlorinator for the polychlorinated aromatic compounds. However, monochlorophenol-dechlorinating anaerobic microorganisms have not been isolated except for few exception. In this study, we have conducted to isolate the monochlorophenol-dechlorinating anaerobic microorganisms. The bio-electrochemical enrichment system, or the enrichment column system with anaerobic plug flow did not enhance the dechlorination of mono-chlorophenol but poly-chlorophenols. Various trials under different conditions suggested that mono-chlorophenol dechlorinating culture did not require other carbon sources such as lactate and formate. Thus, we provided the anaerobic enrichment systems using mono-chlorophenol or phenol as sole source of carbon and energy. The enrichment with phenol resulted in the isolation of anaerobe growing on phenol degradation under sulfate-reducing conditions. The enrichment with 3-chlorophenol was successfully obtained and 3-chlorophenol-dechlorinating anaerobe was identified. These findings expands our knowledge on the mono -chlorophenol dechlorinators and their characteristics.