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Direct analysis of microorganisms involved in the metabolism of multiple aromatic compounds and the production bound residues in soil

直接分析参与多种芳香化合物代谢和土壤中生产结合残留物的微生物

基本信息

批准号：
12680568
负责人：
KATAYAMA Arata
金额：
$ 2.3万
依托单位：
NAGOYA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

This study was conducted to develop the methods (1) to analyze directly the microbial groups metabolizing multiple subtrates in soil based on ^<14>C quinone profile and (2) to analyze directly the chemical structure of bound residues produced during the degradation of aromatic compounds in soil.(1)The microorganisms degrading phenol with the presence of plucose orbenzoic acid were determined by ^<14>C quinone profile method. The microorganisms degrading phenol without other chemicais was mainly the group containing ubiquinone-10 that is alpha-subclass of Proteobacteria. With the presence of glucose, the menaquinone-8(H4) group was the phenol degrader in addition to the ubiquinone-10 group. Menaquinone-8(H4) is contained in Corynebacterineae, Micrococcineae, Propionibacterineae and Pseudonocardineae. With the presence of benzoic acid, the major phenol degraders were not different without benzdc acid These: findmgs has not been obtained. This method enabled the detection of microorganisms degrading a organic compounds with the presence of multiple organic compounds. It will contribute to understand the microbial community in soil. Changes in chemical structures of compounds (^<13>C-labeled rice straw and (^<15>_<13>NH_4)_2SO_4) were measured using ^<13>C-CP/MAS-NMR and ^<15>N-CP/MAS-NMR. Changes in ^<13>C-carbon composition in humic acid during the decomposition of nee straw were directed to that of soil humic acid contained in the S used Changes of nitrogenous compounds were also successfully measured A part of Sen wa, incorporated into fulvic acid that has been considered as inert compounds SgTu^cfducose and a pure culture containing multi microorganisms with a large, the microorganisms degrading glucose was successfully detected. The detection limit of ^<13>C was found relatively low so that the higher labeling levels were required.

本研究旨在开发（1）基于^ C醌谱直接分析土壤中代谢多种底物的微生物类群的方法<14>;（2）直接分析土壤中芳香族化合物降解过程中产生的结合残留物的化学结构的方法。（1）采用~（13）C醌轮廓法对葡萄糖或苯甲酸存在下的苯酚降解菌进行了测定<14>。在没有其他化学物质的情况下降解苯酚的微生物主要是含有泛醌-10的类群，它是变形菌的α亚类。在葡萄糖存在下，甲基萘醌-8（H4）基团是除泛醌-10基团之外的苯酚降解剂。甲萘醌-8（Menaquinone-8，H_4）存在于棒孢霉族、微球菌族、丙酸棒孢霉族和假诺卡氏菌族。在苯甲酸存在下，主要的苯酚降解剂与不存在苯甲酸时没有不同。该方法使得能够在存在多种有机化合物的情况下检测降解有机化合物的微生物。这将有助于了解土壤中的微生物群落。用<13><15><13>~ 1C-CP<13>/MAS-NMR和~ 1 <15>N-CP/MAS-NMR测定了~ 1C标记稻草和（^_NH_4）_2SO_4的化学结构变化。在<13>秸秆分解过程中，腐殖酸中碳碳组成的变化与土壤腐殖酸中碳碳组成的变化有关。同时，还成功地测定了含氮化合物的变化，并成功地检测到一部分被认为是惰性化合物的腐殖酸和一种含有大量葡萄糖降解菌的纯培养物。发现14 C的检测限<13>相对较低，因此需要更高的标记水平。