Direct analysis of microorganisms involved in the metabolism of multiple aromatic compounds and the production bound residues in soil

本研究旨在建立(1)基于^<14>C醌谱直接分析土壤中代谢多种基质的微生物群和(2)直接分析土壤中芳香族化合物降解过程中产生的结合残留物化学结构的方法。(1)采用^<14>C醌谱法测定了在果胶邻苯甲酸存在下降解苯酚的微生物。无其他化学物质降解苯酚的微生物主要是含有泛素-10的类群，是变形菌门的α亚类。当葡萄糖存在时，除泛醌-10组外，甲基萘醌-8（H4）组是苯酚的降解者。甲基萘醌-8（H4）存在于棒状杆菌科、微球菌科、丙酸杆菌科和假心梗菌科。在苯甲酸存在的情况下，主要的苯酚降解剂在没有苯甲酸的情况下没有差异。该方法能够检测存在多种有机化合物的降解有机化合物的微生物。这将有助于了解土壤中的微生物群落。用^<13>C-CP/MAS-NMR和^<15>_<13>NH_4)_2SO_4测定了化合物（^<13 b> C-CP/MAS-NMR）和（^<15> N-CP/MAS-NMR）的化学结构变化。在nee秸秆分解过程中，腐植酸中c -碳组成的变化指向了土壤中所含的S所使用的腐植酸的变化，也成功地测量了含氮化合物的变化。一部分senwa被纳入到被认为是惰性化合物的腐殖酸中，SgTu^cfducose和含有多种微生物的纯培养物中，具有很大的微生物降解葡萄糖。^<13>C的检出限相对较低，因此需要更高的标签水平。

This study was conducted to develop the methods (1) to analyze directly the microbial groups metabolizing multiple subtrates in soil based on ^<14>C quinone profile and (2) to analyze directly the chemical structure of bound residues produced during the degradation of aromatic compounds in soil.(1)The microorganisms degrading phenol with the presence of plucose orbenzoic acid were determined by ^<14>C quinone profile method. The microorganisms degrading phenol without other chemicais was mainly the group containing ubiquinone-10 that is alpha-subclass of Proteobacteria. With the presence of glucose, the menaquinone-8(H4) group was the phenol degrader in addition to the ubiquinone-10 group. Menaquinone-8(H4) is contained in Corynebacterineae, Micrococcineae, Propionibacterineae and Pseudonocardineae. With the presence of benzoic acid, the major phenol degraders were not different without benzdc acid These: findmgs has not been obtained. This method enabled the detection of microorganisms degrading a organic compounds with the presence of multiple organic compounds. It will contribute to understand the microbial community in soil. Changes in chemical structures of compounds (^<13>C-labeled rice straw and (^<15>_<13>NH_4)_2SO_4) were measured using ^<13>C-CP/MAS-NMR and ^<15>N-CP/MAS-NMR. Changes in ^<13>C-carbon composition in humic acid during the decomposition of nee straw were directed to that of soil humic acid contained in the S used Changes of nitrogenous compounds were also successfully measured A part of Sen wa, incorporated into fulvic acid that has been considered as inert compounds SgTu^cfducose and a pure culture containing multi microorganisms with a large, the microorganisms degrading glucose was successfully detected. The detection limit of ^<13>C was found relatively low so that the higher labeling levels were required.