Community analysis of microorganisms metabolizing multiple aromatic compounds in soil

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This study conducted to develop the methods to measure (1) the profile of microbial population degrading aromatic compounds i soil, (2) the microbial groups metabolizing a single substrate in soil using ィイD114ィエD1C quinone profile analysis and (3) the microbial groups metabolizing multiple substrates in soil using ィイD114ィエD1C quinone profile analysis. (1) The profile of microbial population degrading aromatic compounds were determined by using indicator of dehydrogenase activity of microorganisms degrading aromatic compounds and by coloring reaction of chloride anion released from chloroaromatic compounds. The results suggested that application of farmyard manure into soil increase the microbial population to degrade aromatic compounds in soil. The population degrading, 2,4-dichlorophenol in soil was increased by application of farmyard manure but not that degrading 3-chlorophenol. (2) Using glucose, glycine and acetate, ィイD114ィエD1C quinone profile method was developed to detect the microbial groups degrading a single substrate. The reproducibility was high so that the statistical analysis of active microbial community between the soils examined. (3) Phenol and benzoate were used as model aromatic compounds. The ィイD114ィエD1C quinone profile method was used as model aromatic metabolizing phenol in the presence of benzoate and vice versa. Mineralization rates of aromatic compounds and total quinone profiles and labeling rates of quinones were almost identical between the duplicated experiment but the ィイD114ィエD1C quinone profiles were completely different. This suggested that the microenvironment in soil affected the microbial groups metabolizing aromatic compounds in soil.