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Community analysis of microorganisms metabolizing multiple aromatic compounds in soil

土壤中多种芳香族化合物代谢微生物的群落分析

基本信息

批准号：
10680542
负责人：
KATAYAMA Arata
金额：
$ 2.24万
依托单位：
NAGOYA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

This study conducted to develop the methods to measure (1) the profile of microbial population degrading aromatic compounds i soil, (2) the microbial groups metabolizing a single substrate in soil using ィイD114ィエD1C quinone profile analysis and (3) the microbial groups metabolizing multiple substrates in soil using ィイD114ィエD1C quinone profile analysis. (1) The profile of microbial population degrading aromatic compounds were determined by using indicator of dehydrogenase activity of microorganisms degrading aromatic compounds and by coloring reaction of chloride anion released from chloroaromatic compounds. The results suggested that application of farmyard manure into soil increase the microbial population to degrade aromatic compounds in soil. The population degrading, 2,4-dichlorophenol in soil was increased by application of farmyard manure but not that degrading 3-chlorophenol. (2) Using glucose, glycine and acetate, ィイD114ィエD1C quinone profile method was developed to detect the microbial groups degrading a single substrate. The reproducibility was high so that the statistical analysis of active microbial community between the soils examined. (3) Phenol and benzoate were used as model aromatic compounds. The ィイD114ィエD1C quinone profile method was used as model aromatic metabolizing phenol in the presence of benzoate and vice versa. Mineralization rates of aromatic compounds and total quinone profiles and labeling rates of quinones were almost identical between the duplicated experiment but the ィイD114ィエD1C quinone profiles were completely different. This suggested that the microenvironment in soil affected the microbial groups metabolizing aromatic compounds in soil.

本研究旨在建立(1)土壤中降解芳香族化合物的微生物群谱，(2)土壤中代谢单一底物的微生物群谱，(3)土壤中代谢多种底物的微生物群谱，(3)土壤中代谢多种底物的微生物群谱，(3)土壤中代谢多种底物的微生物群谱。(1)利用微生物降解芳香族化合物脱氢酶活性指标和氯芳香族化合物释放的氯阴离子显色反应，确定了降解芳香族化合物的微生物种群概况。结果表明，施用农家肥可增加土壤中降解芳香族化合物的微生物数量。施用农家肥增加了土壤中降解2,4-二氯酚的种群数量，但对降解3-氯酚的种群数量没有影响。(2)以葡萄糖、甘氨酸和乙酸为原料，建立了一种检测降解单一底物的微生物群的方法。重现性高，可用于土壤间活性微生物群落的统计分析。(3)以苯酚和苯甲酸酯为模型芳香族化合物。在苯甲酸盐存在的情况下，用醌谱法模拟芳香代谢苯酚，反之亦然。重复实验间芳香族化合物矿化率、总醌谱和醌类标记率基本一致，但γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ γ。这表明土壤微环境影响了土壤中代谢芳香族化合物的微生物群。