权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis and develerent of micmenviroment in aerobic/anaerobic soils controlling microbial consortia for remediation

好氧/厌氧土壤微生物群落分析及修复控制微生物群落研究

基本信息

批准号：
17310045
负责人：
KATAYAMA Arata
金额：
$ 10.68万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

Soil is a heterogenous environment composed of solid, liquid and atmospheric phases. Microorganisms introduced into soil in the procedure of bioaugumentation for degrading hazardous chemicals are considered to grow only at the microsites with favorable environments. Until now, it has been believed that the growth of soil microorganisms are affected by soil structure of microsites. However, the factors and mechanisms determining the microbial growth at the microsites are not well understood. Especially, the effects of space and physical microenvironment has not been focused. In this project, we evaluate the effect of soil microenvironment, especially physical environment, on microbial growth and the community structure.Microbial biomass and community structure were measured by respiratory quinone profile analysis, direct microscopic method, PCR-DGGE analysis of 16S RNA genes. Microbial activity was measured by dehydrogenase in the case of aerobes and by PCB dechlorination activity in the case of anaerobes.We evaluated the effects of particle size, moisture content and consolidation of soils on the growth and community structure of aerobic microorganisms. Significant effects were observed in particle size and moisture content. Microbial community structure became stable after 1 month of incubation. As model particles, four glass beads with different average diameter (0.01mm to 2mm) were used to make a homogenous physical environment. In aerobic microorganisms, with the decrease of diameter of glass beads, the degree of growth decreased and the ratio of Actinobacteria to Proteobacteria increased. In anaerobic microorganisms, although PCB dechlorination activity was not affected significantly, the different glass beads size resulted in the different community structure.

土壤是由固相、液相和大气相组成的非均质环境。在生物强化降解有害化学品的过程中引入土壤的微生物被认为只能在环境良好的微生境中生长。迄今为止，人们一直认为土壤微生物的生长受到微生境土壤结构的影响。然而，决定微生物生长的因素和机制还没有很好地理解。特别是空间和物理微环境的影响尚未得到重视。本研究采用呼吸醌法、直接显微镜法、16 S RNA基因PCR-DGGE分析等方法，研究了土壤微环境，特别是物理环境对微生物生长和群落结构的影响。微生物的活性测定脱氢酶的情况下，需氧菌和PCB脱氯活性的情况下，厌氧菌。我们评估的颗粒大小，含水量和固结的土壤上的好氧微生物的生长和群落结构的影响。观察到颗粒尺寸和水分含量的显著影响。培养1个月后，微生物群落结构趋于稳定。作为模型颗粒，使用四种不同平均直径（0.01 mm至2 mm）的玻璃珠来制造均匀的物理环境。在好氧微生物中，随着玻璃珠直径的减小，生长程度降低，放线菌与变形菌的比例增加。在厌氧微生物中，虽然对PCB脱氯活性没有显著影响，但不同大小的玻璃珠导致了不同的群落结构。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Anaerobic biodegradation of polychlorinated biphenyls by microbial consortium from uncontaminated paddy soil

未污染稻田土壤中微生物群对多氯联苯的厌氧生物降解

DOI：
发表时间：
期刊：
World Journal of Microbiology and Biotechnology (印刷中)
影响因子：
0
作者：
Daisuke Baba;Tsuyoshi Yasuta;Naoko Yoshida;Yuko Kimura;Katsuhide Miyake;Yasushi Inoue;Koki Toyota;Arata Katayama
通讯作者：
Arata Katayama

A. Effect of particle size on the structure of microbial community in the culture containing glass beads

A. 粒径对含有玻璃珠的培养物中微生物群落结构的影响