Development of an odor discrimination sensor mimicked insect olfactory system

在本研究中，我们旨在建立一种基于昆虫气味受体的高灵敏度和高选择性的细胞气味传感器的方法。利用基因工程技术，我们证明了表达昆虫气味受体和荧光钙指示蛋白的Sf21细胞系可以通过增加荧光强度来敏感地检测一般气味。此外，我们利用微加工技术制作了一个具有两个独立通道的微流控芯片，用于同时提供气味刺激，并证明我们能够测量芯片上平行排列的两个Sf21细胞系的荧光响应。综上所述，我们通过将Sf21细胞系整合到微流控芯片中，建立了开发气味传感器芯片的基本技术。

In this study, we aimed to establish the methodology for developing a cell-based odorant sensor with high sensitivity and good selectivity based on insect odorant receptors. Using genetic engineering technique, we demonstrated that the Sf21 cell lines expressing insect odorant receptors along with a fluorescent calcium indicator protein can sensitively detect general odorants by increasing their fluorescent intensities. In addition, using microfabrication technique we fabricated a microfluidic chip with two independent channels for simultaneously supplying odorant stimuli, and demonstrated that we were able to measure fluorescent responses of two Sf21 cell lines that were arranged in parallel with each other on the chip. Taken together, we established the basic technology for developing an odorant sensor chip by integrating the Sf21 cell lines into the microfluidic chip.