Establishment of a novel method to identify the hydroxymethylcytosine in single base resolution.

DNA甲基化是哺乳动物基因组系统表观遗传调控的重要修饰之一。最近发现了甲基胞嘧啶氧化成羟甲基胞嘧啶的反应。因此，建立一种在单碱基分辨率下鉴定基因组羟甲基胞嘧啶的方法具有重要意义。本文提出了一种利用DNMT1酶鉴定甲基胞嘧啶和羟甲基胞嘧啶的新方法，具有较高的准确度（95%以上）。

DNA methylation is one of the important modifications in epigenetic regulation of mammalian genome system. Recently, the oxidation reaction of methylcytosine to hydroxymethylcytosine is discovered. Thus, it is important to establish a method to identify the hydroxymethylcytosine of the genome in single base resolution. Here I provide a novel method to identify the methylcytosine and the hydroxymethylcytosine with high accuracy (more than 95%) using DNMT1 enzyme.