Proteomic identification of dephosphorylated proteins by phosphatase protein

蛋白激酶和磷酸酶的蛋白磷酸化和去磷酸化调节植物中的许多生物事件。为了鉴定尽可能多的磷酸蛋白，人们已经建立了几种磷酸肽的纯化方法。在此，我们使用了两种磷酸肽纯化柱(PhosTio和PolyMac)，每种方法都可以从水稻培养细胞中鉴定出771和641个磷酸肽。在稻瘟病菌处理的水稻培养细胞中，鉴定出913和802个磷酸肽。这些结果表明，几种蛋白的磷酸化被诱导来响应病原菌的攻击，并可能在抗病中发挥作用。

Protein phosphorylation and dephosphorylation by protein kinases and phosphatases regulate many biological events in plants. To identify as many phosphoproteins as possible, several purification methods of phosphopeptides have been established. Here, we used two kind of phosphopeptides purification columns (PhosTio and PolyMac) and each method allowed identifying 771 and 641 phosphopeptides in rice cultured cells. Also, 913 and 802 phosphopeptides were identified in rice cultured cells treated with rice blast fungus. These results indicate that phosphorylation of several proteins are induced responded to pathogen attack and may have a role in disease resistances.